Article Sidebar
Views PDF: 165
DEVELOPMENT OF AN LC/MS/MS METHOD FOR DETERMINATION SALBUTAMOL, RACTOPAMNE AND CLENBUTEROL RESIDUES IN CHICKEN MEAT
Main Article Content
Abstract
In this study, we developed a rapid and accurate quantitative analysis of Salbutamol, Ractopamine and Clenbuterol residues in poultry meat by UPLC-MS/MS. Conditions of mass spectrometry include chromatographic column: C18; 100×2,1mm; 1.7µm, mobile phase: ACN (0.1% Formic Acid): 0.1% Formic Acid in water (appropriate ratio), flow rate: 0.2mL/min, injection volume: 1µL. Our method presented satisfactory results in terms of sensitivity, precision, accuracy, and recovery of quantitatively analyzing simultaneous Salbutamol, Ractopamine and Clenbuterol residues in poultry meat. This method has been proved to be fast, reliable and reproducible to accurately analyze Salbutamol, Ractopamine and Clenbuterol.
Article Details
Keywords
Salbutamol, Ractopamine, Clenbuterol, LC/MS/MS, poultry
References
2. Jaekyu Shin., Daniel F. Pauly., Julie A. Johnson., Reginal F. Frye (2008), “Simplified method for determination of clarithromycin in human plasma using protein precipitation in a 96 - well format and liquid chromatography - tandem mass spectrometry”, Jounal of Chromatography B 871, pp.130 - 134.
3. Xiumei Lu., Lingyun Chen., Dong Wang., Juan Liu., Yanjuan Wang., Famei Li (2008), “Quantification of Clarithromycin in Human Plasma by UPLC - MS - MS”, Shenyang Pharmaceutical University, June 2008.
4. Syed N Alvi., Saleh Al Dgither and Muhamad M Hammami (2016), “Rapid Determination of Clarithromycin in Human Plasma by LCMS/MS Assay”, Pharmaceutical Analytical Chemistry Open Access, pp 2471 - 2695.
5. Guidance for industry - Bioanalytical method validation, FDA 2018.
6. Guidance on Bioanalytical method validation, EMA 2012.