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Date Published: 25/04/2022
Abstract Views: 216
Views PDF: 183
DOI: https://doi.org/10.51298/vmj.v512i1.2225
Issue
Vol. 512 No. 1 (2022)
Section
Các bài báo
How to Cite
Tiến Trường, Đặng ., Ngọc Hà, Q., Văn Quyết, P. ., & Duy Bắc, N. . (2022). RESEARCH ON EFFICIENCY RECOVERY OF CFFDNA OF TWO COMMERCIAL DNA KITS. Vietnam Medical Journal, 512(1). https://doi.org/10.51298/vmj.v512i1.2225

RESEARCH ON EFFICIENCY RECOVERY OF CFFDNA OF TWO COMMERCIAL DNA KITS

Đặng Tiến Trường1, Quản Ngọc Hà1, Phạm Văn Quyết1, Nguyễn Duy Bắc1,
1 Vietnam Military Medical University

Main Article Content

Abstract

Objective: To evaluate the efficiency of cell free fetal DNA (cffDNA) recovery of 2 commercial kits QIAamp Circulating Nucleic Acid and MagMAX Circulating DNA. Subjects and research methods: plasma samples of pregnant women with male fetuses. The study compared the efficiency of cfDNA recovery of two extraction procedures based on fetal fraction (FF) by quantitative real-time PCR. Result: The average FF of the QIAamp kit was 7.93%; The average FF of the MagMAX kit was 6.23%. The mean FF of QIAamp was 1.27 times higher than that of MagMAX, but the difference was not significant (p=0.33). Conclusion: There was no difference in the cffDNA recovery of the QIAamp kit and the MagMAX kit.

Article Details

Keywords

cfDNA, cffDNA, MagMAX and QIAamp

References

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