KHẢO SÁT SỰ ỨC CHẾ TỔNG HỢP SẮC TỐ MELANIN TRÊN DÒNG TẾ BÀO U HẮC TỐ B16F10 CỦA CAO CHIẾT VỎ KHÓM (Ananas Comosus (L) Merr.) THU HÁI Ở VÙNG TẮC CẬU, KIÊN GIANG

Abstract

Pineapple is a fruit with high nutritional value. The purpose of this study was to investigate the inhibition of melanin synthesis on the B16F10 cell line of the pineapple peel methanol extract. Using morphological analysis method combined with sequencing to identify pineapple species collected in Tac Cau, Kien Giang province, Evaluation of antioxidant capacity by determining DPPH free radical neutralization, Fe³⁺ ion reduction and Cu²⁺ ion reduction. Investigation of the ability to inhibit tyrosinase enzym activity in vitro and inhibit melanin formation on the melanoma cell line B16F10. Result, the pineapple sample collected in Tac Cau, Kien Giang province has the scientific name Ananas comosus (L.) Merr. Ability to reduce DPPH, Fe³⁺, Cu²⁺ of pineapple peel through IC₅₀ respectively index are 62.63 µg/mL, 97.72 µg/mL, 220.95 µg/mL; nhibited tyrosinase enzym activity in vitro with an IC₅₀ is 73.13 g/mL; Inhibition of melanin production on the B16F10 cell line reached 59.37% at a concentration of 10 µg/mL. The research has initially shown that the methanol extract of pineapple peel is a potential source of raw materials in the field of pharmaceutical and cosmetic production.