MỐI LIÊN QUAN GIỮA ĐA HÌNH GEN METHYLENE TETRAHYDROFOLATE REDUCTASE (MTHFR) C677T VỚI LOÃNG XƯƠNG Ở NAM GIỚI

Abstract

Previous studies have shown that homocysteine level and the C677T polymorphism in the gene encoding Methylenetetrahydrofolate reductase (MTHFR) may be involved in thedevelopment of osteoporosis in Asian population. The aim of this study was to verify the association of thispolymorphism with osteoporosis in older Vietnamese men.A cohors study on 400 men aged 50 years and over (200 osteoporosis and 200control). BMD at lumbar spine 1–4 and the left proximal femur including the femoral neck and the total hip were measured by dual-energy X-ray absorptionmetry in all subjects. MTHFR C677T genotypes were determined by Amplifications Refractory Mutation System – Polymerase chain reaction (ARMS-PCR) Results: The frequency of genotype CC/CT/TT of the study group was: 62.5%/33.5%/4.0%, respectively. The frequencies of C and T alleles are (79.25%) and (20.75%), respectively, the distribution of C and T alleles follows the Hardy-Weinberg law. In which, CT and TT genotypes appeared in the disease group (36.5% and 6%) higher than in the control group (30.5% and 2%) the difference was statistically significant (p=0.036); CT/TT genotype carriers have an increased risk of osteoporosis 1.54 times compared with CC genotype carriers (95% CI: 1.02-2.31), in which the femoral neck position is 2.04 times (95%CI: 1.23-3.38); after testing multivariate logistic regression with risk factors ages, BMI, history of smoking, alcohol consumption, history of fracture, physical activity showed that the CT/TT genotype increased the risk of osteoporosis by more than 1,52 times compared with the CC genotypebut not statistically significant. Conclusion: Our data show that the MTHFR C677T polymorphism is an independent predictor of osteoporosis in men.