XÂY DỰNG VÀ THẨM ĐỊNH PHƯƠNG PHÁP PHÂN CỰC NGHIỆM ĐỊNH LƯỢNG CHẤT HOẠT QUANG VỚI THỂ TÍCH MẪU NHỎ

Abstract

A polarimetric method for the quantification of the optically active compounds Glucose and Sucrose with small sample volume has been developed and validated. Using a quartz cuvette with a volume of about 1 ml to replace a conventional polarizer with a volume of 10 ml reduces the volume of the standard and test sample by 10 times. Glucose and sucrose are investigated in the concentration range of 4- 40 % w/v. Regression statistics of Glucose is 0,9999 for coefficient of determination, the limit of detection (LOD) and limit of quantitation (LOQ) are found to be 0,78 and 2,36 %w/v. Regression statistics of Sucrose is 0,9998 for Coefficient of determination, the LOD and LOQ are found to be 0,95 and 2,89 % w/v. The method employed quartz cuvette instead of a polarimeter tube is verified in laboratory and is applied for two samples whose have Glucose and Sucrose in the market.