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Date Published: 23/09/2022
Abstract Views: 623
Views PDF: 337
DOI: https://doi.org/10.51298/vmj.v518i1.3378
Issue
Vol. 518 No. 1 (2022)
Section
Các bài báo
How to Cite
Đức Trưởng, N. ., Thị Thùy Dương, Đặng ., Huy Hoàng, L. ., Thùy Trâm, N., Thị Nga, T. ., Bảo Yên, P. ., & Hồng Quân, D. . (2022). COMPARISON OF LAMP TECHNIQUE WITH ISOLATION CULTURING PROCEDURE FOR TYPE A, B ISOLATION OF CLOSTRIDIUM BOTULINUM GENES IN FOOD SAMPLES AND CLINICAL DISEASES. Vietnam Medical Journal, 518(1). https://doi.org/10.51298/vmj.v518i1.3378

COMPARISON OF LAMP TECHNIQUE WITH ISOLATION CULTURING PROCEDURE FOR TYPE A, B ISOLATION OF CLOSTRIDIUM BOTULINUM GENES IN FOOD SAMPLES AND CLINICAL DISEASES

Nguyễn Đức Trưởng1,, Đặng Thị Thùy Dương2, Lê Huy Hoàng3, Nguyễn Thùy Trâm3, Tăng Thị Nga3, Phạm Bảo Yên4, Dương Hồng Quân5
1 Haiduong Provincial General Hospital
2 Haiduong Medical Technical University
3 National Institute of Hygiene and Epidemiology
4 Vietnam National University - University of Science
5 Trường Đại học Y tế công cộng

Main Article Content

Abstract

Objectives: The study compares the LAMP technique with the culture technique to detect type A and B toxin genes of Clostridium botulinum (C. botulinum). Subjects and research methods: Experimental study in the laboratory tested on 90 food samples and clinical specimens. Results: Use the results of the isolation culture to detect the toxin gene as a standard for comparison with the LAMP procedure. The study results showed that the number of samples showing positive results when performing the LAMP procedure to detect type A and B toxin genes of C. botulinum bacteria was 12/90 (13,3%) while the stool culture procedure The established toxin gene was 11/90 (12,2%). The sensitivity, specificity, and accuracy (accuracy) of the LAMP procedure to detect the type A and B toxin genes of C. botulinum were 91.6%, 100%, and 98,8%, respectively. Besides, the false-positive rate was 8,33%, the false-negative rate was 0%, especially the kappa coefficient was 0,986, showing an almost complete consensus between the two procedures. In addition, the LAMP technique shows many advantages such as faster implementation time, more cost savings, easier to implement, and can be deployed in all laboratories from small to large to detect type toxins. A, B of C. botulinum bacteria in food as well as clinical samples.

Article Details

Keywords

Botulinum toxin, Clostridium botulinum, LAMP, Meat poisoning

References

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