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Date Published: 09/10/2022
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DOI: https://doi.org/10.51298/vmj.v518i2.3462
Issue
Vol. 518 No. 2 (2022)
Section
Các bài báo
How to Cite
Thái Khương, L. ., Quốc Chương, H. ., Bích Trâm, D. ., & Anh Vũ, H. . (2022). APPLICATION OF SANGER SEQUENCING TO DETECT MITOCHONDRIAL DNA VARIANTS. Vietnam Medical Journal, 518(2). https://doi.org/10.51298/vmj.v518i2.3462

APPLICATION OF SANGER SEQUENCING TO DETECT MITOCHONDRIAL DNA VARIANTS

Lê Thái Khương1, Hồ Quốc Chương1, Dương Bích Trâm1, Hoàng Anh Vũ1,2,
1 Center For Molecular Biomedicine, University of Medicine and Pharmacy at Ho Chi Minh City
2 University of Medicine and Pharmacy at Ho Chi Minh City

Main Article Content

Abstract

Aim: Mitochondria play a critical role in the generation of metabolic energy in eukaryotic cells. Mitochondrial DNA is assumed to experience a higher mutation rate than nuclear DNA and mitochondrial DNA mutation is one of the major causes of human diseases. This study aims to detect mitochondrial DNA mutations using Sanger sequencing technique. Materials and methods: Mitochondrial DNA was extracted from peripheral blood samples of patients with mitochondrial disorders. PCR and Sanger sequencing were thereafter established to identify mutations on mitochondrial DNA. Results: There were 19 cases carrying mitochondrial DNA variants among a total of 43 cases, in which m.3243A>G mutation accounted for the highest rate (73.68%). Conclusion: Detection of mitochondrial DNA variants has been successfully and effectively established via utilization of Sanger sequencing technique.

Article Details

Keywords

Mitochondrial disorders, mitochondrial DNA, Sanger sequencing

References

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