XÂY DỰNG QUY TRÌNH CHIẾT CAO ĐỊNH CHUẨN KIỂM SOÁT HÀM LƯỢNG NUCIFERIN TỪ LÁ SEN (NELUMBO NUCIFERA GAERTN) THU HÁI TẠI ĐỒNG THÁP

Abstract

Background: Lotus is one of the few herbs in which all parts are used and are precious medicinal herbs with high biological values that can find in all provinces of the country, and the most famous is in Dong Thap Muoi, considering the land of Sen. The pharmacological studies have proved that the nuciferine component in Lotus leaf extract has many biological activities in vivo such as insomnia, antihyperlipidemic, inhibiting the growth of cancer cells, and antioxidant effects. Currently, there are many preparations made from the raw materials of Lotus leaves. However, the control of nuciferine components related to anti-hyperlipidemia biological effects in these preparations has not been done, significantly impacting product safety and effectiveness. Objectives: Develop a standardized extraction procedure to control nuciferine content from Lotus leaves collected in Dong Thap province. Materials and methods: Lotus leaf collected in Dong Thap province met the criteria of testing for medicinal herbs regarding moisture, quality, and quantity of Vietnamese Pharmacopoeia V. We used the cold soaking method to examine the extraction conditions (extraction solvent, time, the number of extraction times, the amount of solvent) and the process of removing impurities. The controlled extract of nuciferine was determined by utilizing high-performance liquid chromatography coupled with a photodiode arrays detector (HPLC-PDA). Results: The optimum extraction parameter include 70^o alcohol, three times of extraction, material-solvent ratio (1:10). From 5 kg of dried lotus leaf to obtain 80,5g of controlled extract of nuciferine with 10% of nuciferine were determined by HPLC/PDA method. Conclusion: This study successfully developed a standardized extraction procedure with controlled nuciferine content from Folium Nelumbinis collected in Dong Thap province at the 5 kg of dried leaves. This proposed process can implement on an industrial scale to produce safe and effective products.