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Date Published: 13/02/2023
Abstract Views: 669
Views PDF: 426
DOI: https://doi.org/10.51298/vmj.v521i1.3949
Issue
Vol. 521 No. 1 (2022)
Section
Các bài báo
How to Cite
Nguyễn, T. A., Nguyễn, M. T., Lê, T. T. T., Phan, C. T., Nguyễn, T. N. Y., Nguyễn, H., Trần, Q. V., & Tôn, H. D. (2023). COMPARISON AND EVALUATION OF MULTIPLEX PCR TECHNIQUE PROCESS OF THE IDENTIFICATION CANDIDA SPP. FROM PATIENT SAMPLES. Vietnam Medical Journal, 521(1). https://doi.org/10.51298/vmj.v521i1.3949

COMPARISON AND EVALUATION OF MULTIPLEX PCR TECHNIQUE PROCESS OF THE IDENTIFICATION CANDIDA SPP. FROM PATIENT SAMPLES

Tú Anh Nguyễn1, Minh Thái Nguyễn2, Thị Thanh Thảo Lê2, Cảnh Trình Phan2, Thị Ngọc Yến Nguyễn3, Hiếu Nguyễn1, Quốc Việt Trần4, Hoàng Diệu Tôn5,
1 University of Medicine and Pharmacy at Ho Chi Minh City
2 niversity of Medicine and Pharmacy at Ho Chi Minh City
3 Nguyen Tat Thanh University
4 Military Hospital 175
5 Pham Ngoc Thach University of Medicine

Main Article Content

Abstract

Background: Although traditional methods of detecting species of Candida are easy to implement, the disadvantages are dependent on objective factors and are time-consuming. The simple methods that can quickly detect Candida spp. with high reliability, specificity, and especially, simultaneous detection of many pathogenic species in the patient samples being researched and developed – the multiplex PCR. Objectives: This study aimed to (1) Detect of 4 species of C. albicans, C. glabrata, C. tropicalis, and C. parapsilosis by multiplex PCR and traditional methods. (2) Compare and evaluate the process of detecting 4 species Candida spp. from patient samples by multiplex PCR technique. Method: Sample Candida spp. was admitted 3 hospitals in Ho Chi Minh city from October 2020 to May 2021. The fungus was detected by 3 methods: (1) Germ tube, (2) isolation on CHROMagar Candida medium, and (3) multiplex PCR technique. Then, compare the detection process of 4 species of Candida by multiplex PCR technique and detection method on CHROMagar Candida, based on sensitivity, specificity, positive predictive value, negative predictive value, repeatability, and accuracy. Results: Isolated on CHROMagar Candida medium: 186 isolates with the highest infection rate being C. albicans 112/186 (55.45%), C. tropicalis 39/186 (19.31%), C. glabrata or C. parapsilosis 35/189 (17.33%) and 16 samples suspected of not belonging Candida. Identification by multiplex PCR: 186 PCR products were detected, of which C. albicans 112/186 (55.45%), C. tropicalis 39/186 (19.31%), C. glabrata 25/ 186 (12.38%), C. parapsolosis 10/189 (4.59%) and 16 samples did not detect PCR products. Multiplex PCR procedure detected 4 species of Candida spp. The criteria were evaluated, and there were 5 satisfactory criteria according to the guidance of the Ministry of Health (2016): sensitivity (93.33%), specificity (100%), accuracy (96.19%), positive predictive value (100%), repeatability (pass), negative predictive value (66.67%) < 90%.

Article Details

Keywords

Candida spp.; Multiplex PCR.

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