NGHIÊN CỨU TÁC DỤNG SINH CYTOKIN CỦA KHỐI TẾ BÀO CAR-T

Abstract

Objectives: The study on IL-2, TNF-α and IFN secretion of CAR-T cell when co-incubation CD19 + cancer cell lines. Method: Interleukin generation of CAR-T cell when co-culture with other cells (K562, Daudi, 1D2, PBMC cells) was evaluated at 24 and 48 hours after culture. IL-2, TNF-α and IFN were quantified using the probiotics IL-2, TNF alpha, IFN gamma Human ELISA Kit (Thermo Fisher Scientific). Results: At both 24 and 48 hours, the higher concentrations of cytokines IFN-γ, IL-12, and TNF-α in the CAR-T cell cultures when cultured with cancer cells with CD19 + statistical significance (p <0.05) compared with the concentration of IFN-γ, IL-12, TNF-α in the culture of above cells and PBMC cells (control group). In addition, the concentrations of these cytokines in CAR-T cell cultures were both higher when cultured with CD19 + cancer cells than when cultured with CD19- cells. Conclusion: Our CAR-T product has the ability to generate IFN-γ, IL-12, TNF-α and increase generation when co-cultured with CD19 positive cells.