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ANALYSIS OF GLUCOSE-6-PHOSPHATE DYHYDROGENASE DEFICIENCY GENETIC VARIANTS BY MULTIPLEXED HIGH-RESOLUTION TECHNIQUE IN MALAARIA ENDEMIC ZONE OF DAK NONG PROVINCE
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Abstract
Glucose-6-phosphate dehydrogenase (G6PD) deficiency is an X-linked erythrocyte enzyme disorder with relevance to Plasmodium vivax malaria treatment policy. Treatment with the antimalarial primaquine (PQ) can result in hemolytic anemia in G6PD deficient patients, hence using full-dose in PQ for fostering vivax malaria elimination, need to identify G6PD variants to inform rational treatment policy. Method: A cross-sectional study in field and laboratory-based molecular analysis on 2,809 people was conducted using a quantitative CareStart™ G6PD biosensor (AccessBio, USA) and PCR and Sanger sequencing for finding of the G6PD mutations. Results: The results showed that the population overall proportion of G6PD deficiency was 2,31%, in which male and female were 3.65% and 1.49%, respectively. The prevalence of G6PD deficiency was significantly different among ethnic minority groups (p<0.005). For G6PD genotyping, Viangchan was highest detected in 89.23% (58/65), next to Mahidol was 6.15% (4/65), and a new G6PD Canton was 4.62% (3/65), whereas other G6PD variants were absent. Conclusions: The quantitative test should include point-of-care G6PD activity testing in clinical practice for vivax malaria radical treatment, and the discovery of G6PD variants contributes to proper treatment policy.
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Keywords
G6PD deficiency, Plasmodium vivax, CareStart™ G6PD biosensor.
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