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Date Published: 15/02/2023
Abstract Views: 336
Views PDF: 221
DOI: https://doi.org/10.51298/vmj.v521i2.4056
Issue
Vol. 521 No. 2 (2022)
Section
Các bài báo
How to Cite
Phạm , T. H., Trần, T. C. M., Bùi , T. N. H., & Nguyễn, T. H. . (2023). VALIDATION OF PLASMA COPPER MEASUREMENT BY GRAPHITE FURNACE ATOMIC ABSORPTION SPECTROPHOTOMETRY. Vietnam Medical Journal, 521(2). https://doi.org/10.51298/vmj.v521i2.4056

VALIDATION OF PLASMA COPPER MEASUREMENT BY GRAPHITE FURNACE ATOMIC ABSORPTION SPECTROPHOTOMETRY

Thu Hiền Phạm 1,, Thị Chi Mai Trần1,2, Thị Ngọc Hà Bùi 3, Thị Huệ Nguyễn4
1 Vietnam National Children's Hospital
2 Hanoi medical university
3 Hanoi University of Public Health
4 Vietnam National Children's Hospital.

Main Article Content

Abstract

SPECTROPHOTOMETRY


Objectives: The aim of this study was to develop and validate the plasma copper quantitation method by graphite furnace atomic absorption spectrophotometry. Method: The limit of detection, limit of quantitation, linear range, precision and accuracy of the plasma copper quantitation by GFAAS were evaluated. Results: The LOB, LOD and LOQ of this method were 0.44 µmol/L, 1.06 µmol/L and 2µmol/L respectively. The method linearity was from 2 to 50 mmol/L. The repeatability at the concentrations of 11.7 and 21.3 (mmol/L) were 3.09 and 2.14 (%) respectively. The reproducibility at the two concentrations above were 6.68 and 4.68 (%) respectively. The recovery of spiked samples were 108.5; 102.8; và 94.3 %,  fell in range of  80-110%;  acceptable following the AOAC 2016 criteria. Conclusion: The developed direct method for plasma copper quantitation by GFAAS was accurate and precise, can be used for diagnosis and treatment monitoring of copper metabolism disorders.

Article Details

Keywords

Method validation, Plasma copper measurement, graphite furnace atomic absorption spectrophotometry.

References

1. Kaler, Stephen G. (1998). "Metabolic and molecular bases of Menkes disease and occipital horn syndrome". Pediatric and Developmental Pathology. 1 (1): 85–98.
2. Nagral A, Sarma MS, Matthai J, Kukkle PL, Devarbhavi H, Sinha S, Alam S, Bavdekar A, Dhiman RK, Eapen CE, Goyal V, Mohan N, Kandadai RM, Sathiyasekaran M, Poddar U, Sibal A, Sankaranarayanan S, Srivastava A, Thapa BR, Wadia PM, Yachha SK, Dhawan A. Wilson's Disease: Clinical Practice Guidelines of the Indian National Association for Study of the Liver, the Indian Society of Pediatric Gastroenterology, Hepatology and Nutrition, and the Movement Disorders Society of India. J Clin Exp Hepatol. 2019 Jan-Feb;9(1):74-98.
3. Ward AF, Mitchell DG, Kahl M and Aldous KM. Determination of copper in plasma and serum by use of a microsampling cup in atomic absorption spectrometry. Clin Chem 1974, 20 (9): 1199-1203.
4. Tan M, Sudjadi, Astuti, Rohman. Validation and quantitative analysis of cadmium, chromium, copper, nickel, and lead in snake fruit by Inductively Coupled Plasma-Atomic Emission Spectroscopy. Journal of Applied Pharmaceutical Science 2018, 8(2):044-048.
5. Ramos NC, Lamorena RB. Detection of copper, cadmium, manganese, lead and zinc content in milled rice using microwave plasma atomic emission spectroscopy. Philipine journal of Science 2021, 150(4): 765- 776.
6. MCGahan MC and Bito LZ. Determination of copper concentration in blood plasma and in ocular and cerebrospinal fluids using graphite furnace atomic absorption spectroscopy. Analytical Biochemistry 1983, 135: 186- 192.
7. RobeLourt NB, Faircluogh D, McLoughlin S and Taylor WH. Measurement of copper, zinc and magnesium in serum and urine by DC plasma emission spectrometry. Ann Clin Biochem 1985, 22: 533- 538.