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Date Published: 15/02/2023
Abstract Views: 373
Views PDF: 252
DOI: https://doi.org/10.51298/vmj.v521i2.4105
Issue
Vol. 521 No. 2 (2022)
Section
Các bài báo
How to Cite
Nguyễn, T. N. Ái, & Đặng, T. N. D. (2023). PERFORMANCE VERIFICATION OF REALTIME RT-PCR FOR SARS-CoV-2 DETECTION. Vietnam Medical Journal, 521(2). https://doi.org/10.51298/vmj.v521i2.4105

PERFORMANCE VERIFICATION OF REALTIME RT-PCR FOR SARS-CoV-2 DETECTION

Thị Như Ái Nguyễn1,, Thị Ngọc Dung Đặng2
1 Viet Tiep Hospital
2 Hanoi medical university

Main Article Content

Abstract

Recently, Realtime RT-PCR is considered one of the best methods to detect SARS-CoV-2. Because of complex specifications, Realtime RT-PCR assays require verification and rigorous quality control. Object: Verification of the use value of the Allplex™ SARS-CoV-2-Seegenee kit at the department of Biochemistry laboratory of Vietnamese-Czech hospital, Hai Phong to ensure accuracy and reliability Subjects and Methods: Verifying the parameters of precision,and  accuracy of Allplex™ SARS-CoV-2-Seegenee kit according to the recommendations of the American Society for Microbiology (ASM). Results: The assay precision reached CV% values ​​for short-term and long-term precision in accordance with the manufacturer's clamis. The accuracy of the positive and negative consensus test reached 100%. Conclusion: method verification is in accordance with the manufacturer's claims.

Article Details

Keywords

SARS-CoV-2, verification, Realtime RT-PCR, Verification and validation, ASM

References

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