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ANTIBIOTIC RESISTANT CHARACTERISTICS OF ESCHERICHIA COLI HARBORING MCR-1 STRAINS ISOLATED FROM HEALTHY RESIDENTS IN THAI BINH
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Abstract
Escherichia coli harboring mcr-1 in stool samples of healthy residents in Thai Binh and analyse antibiotic resistant characteristics of these strains. A total of 612 stool samples were collected from healthy residents in Nguyen Xa, Vu Thu, Thai Binh during the period from 2013 to 2016. These stool samples were cultured on MacConkey agar supplemented with cefotaxime 1 mg/L. For each sample, one representative E. coli-like colony was isolated, identified by biochemical tests and evaluated for susceptibility to 14 antibiotics, then the minimum inhibitory concentration (MIC) with colistin was determined according to the CLSI guideline. The presence of mcr-1 gene in E. coli were determined by Real-time PCR method. The result indicated that the prevalence of E. coli harboring mcr-1 in healthy residents in Thai Binh was 5.1%. Result of susceptibility tests revealed that 100% of E. coli harboring mcr-1 strains were multidrug resistant bacteria and 96.8% of them were non-susceptible to five to eight antibiotic classes. These strains were resistant to many common antibiotics with rates as high as 51.6 to 100%, but the resistance rates to cefoxitin, fosfomycin, ceftazidime and meropenem were quite low (<10%). All of these strains (100%) were resistant to colistin (MIC ≥ 4 mg/L). The study showed that multidrug resistant E. coli harboring mcr-1 was widely spread among healthy residents in Thai Binh. Therefore, appropriate interventions should be implemented to prevent the spread of multidrug-resistant bacteria in the community.
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Keywords
E. coli, multidrug resistance, colistin-resistance, mcr-1.
References
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