Article Sidebar

PDF
Date Published: 07/03/2023
Abstract Views: 300
Views PDF: 335
DOI: https://doi.org/10.51298/vmj.v523i2.4548
Issue
Vol. 523 No. 2 (2023)
Section
Các bài báo
How to Cite
Hoàng, A. V. ., Phạm , N. L. ., Nguyễn, N. P. D. ., Diệp , H. V., Trương , M. N. ., & Nguyễn, H. L. T. (2023). STIMULTANEOUS DETERMINATION OF NARINGIN AND HESPERIDIN IN YOUNG POMELO POWDER BY LIQUID CHROMATOGRAPHY TANDEM MASS SPECTROMETRY (LC-MS/MS). Vietnam Medical Journal, 523(2). https://doi.org/10.51298/vmj.v523i2.4548

STIMULTANEOUS DETERMINATION OF NARINGIN AND HESPERIDIN IN YOUNG POMELO POWDER BY LIQUID CHROMATOGRAPHY TANDEM MASS SPECTROMETRY (LC-MS/MS)

Anh Việt Hoàng1, Ngọc Liên Phạm 1, Ngọc Phương Diễm Nguyễn2, Hoàng Vũ Diệp 2, Minh Nhựt Trương 1, Hữu Lạc Thủy Nguyễn1,
1 University of Medicine and Pharmacy at Ho Chi Minh City
2 Institute of drug quality control Ho Chi Minh city

Main Article Content

Abstract

Objectives: Stimultaneous determination the content of naringin and hesperidin in young pomelo. Subjects and Methods: naringin an hesperidin in young pomelo. The qualitative procedure by HPLC-MS/MS was built and validated. Results: Chromatographic conditions: C8 column, mobile phase methanol – acetic acid 0.1 % (50 : 50), flow rate 0.6 ml/min, column temperature 30 oC, autosampler temperature 10oC, injection volume 5 μl, and MS/MS detector with ESI (-), MRM mode. Validation results proved the proposed method was suitable for determining the content of naringin and hesperidin in young pomelo powder. Conclusion: The procedure can be applied for stimultaneous determination the content of naringin and hesperidin in young pomelo.

Article Details

Keywords

naringin, hesperidin, young pomelo, LC-MS/MS.

References

1. Anmol RJ, Marium S. et al., “Phytochemical and Therapeutic Potential of Citrus grandis (L.) Osbeck: A Review”, J Evid Based Integr Med, 2021:12-15.
2. AOAC International, “AOAC Guidelines for Single Laboratory Validation of Chemical Methods for Dietary Supplements and Botanicals”, 2002;Section 3.4:17-22.
3. Chen L, Lai Y, Dong L, Kang S, Chen X., “Polysaccharides from Citrus grandis (L.) Osbeck suppress inflammation and relieve chronic pharyngitis”, Microbial pathogenesis, 2017;113:365-371.
4. ICH Harmonised Tripartite Guideline, “Validation of analytical procedures: text and methodology”, 2015:1-13.
5. Mäkynen K, Jitsaardkul S, Tachasamran P, et al., “Cultivar variations in antioxidant and antihyperlipidemic properties of pomelo pulp (Citrus grandis (L.) Osbeck) in Thailand”, Food Chem, 2013;139(1-4):735-743.
6. Xi W., Fang B., Zhao Q. et al., “Flavonoid composition and antioxidant activities of Chinese local pummelo (Citrus grandis Osbeck.) varieties”, Food Chem, 2014;161:230-238.
7. Yusof S., Ghazali H. M., King G. S. J. F. C., “Naringin content in local citrus fruits”, Food Chem, 1990;37(2):113-121.
8. Yuting C., Rongliang Z., Zhongjian J. et al., “Flavonoids as superoxide scavengers and antioxidants”, Free Radical Biology and Medicine, 1990;9(1):19-21.