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Date Published: 17/03/2023
Abstract Views: 299
Views PDF: 178
DOI: https://doi.org/10.51298/vmj.v524i1A.4613
Issue
Vol. 524 No. 1A (2023)
Section
Các bài báo
How to Cite
Vũ , T. H. ., Nguyễn, H. V. ., Trần , T. H. T. ., Luyện , T. T. N., Đoàn, T. K. P., Lương, T. L. A. ., Nguyễn, H. B. ., Trần , Đức P. ., & Nguyễn, N. H. . (2023). EVALUATING THE mRNA EXPRESSION LEVEL OF SPAG8 GENE IN MALE INFERTILITY. Vietnam Medical Journal, 524(1A). https://doi.org/10.51298/vmj.v524i1A.4613

EVALUATING THE mRNA EXPRESSION LEVEL OF SPAG8 GENE IN MALE INFERTILITY

Thị Hà Vũ 1,2,, Hoàng Việt Nguyễn1, Thị Huyền Trang Trần 1, Thị Thanh Nga Luyện 3, Thị Kim Phượng Đoàn1,2, Thị Lan Anh Lương1,2, Hoài Bắc Nguyễn2, Đức Phấn Trần 1,2, Ngân Hà Nguyễn1
1 Hanoi medical university
2 Hanoi medical university hospital
3 Medlatec hospital

Main Article Content

Abstract

The regulation of spermatogenesis and sperm differentiation is controlled by different types of gene. SPAG8 (sperm associated antigen 8) is a gene involving in the regulation process but the role of this gene remains unclear. Objectives: Evaluating the mRNA expression level of SPAG8 gene in blood samples from infertile males. Material and methods: total RNA from 50 peripheral blood samples (25 samples of infertile males, 25 samples of control group) were extracted. The mRNA expression level of SPAG8 gene were determined using RT-qPCR assay. Results: The average mRNA expression level of SPAG8 gene in infertile males = 0.8205±1.316 (0.091 – 3.977). The average mRNA expression level of SPAG8 gene in the control group = 1,418±1,115 (0.306 – 3.464). The mRNA expression level of the SPAG8 gene of the male infertility group was lower than the control group, the difference was statistically significant with p<0.05. Conclusion: Initial study has shown that the expression level of SPAG8 gene in peripheral blood is lower in male infertility patients than in control group, suggesting a correlation between this gene and male infertility.

Article Details

Keywords

Male infertility, mRNA, RT-qPCR, SPAG8

References

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