Article Sidebar

PDF
Date Published: 17/03/2023
Abstract Views: 540
Views PDF: 529
DOI: https://doi.org/10.51298/vmj.v524i1A.4644
Issue
Vol. 524 No. 1A (2023)
Section
Các bài báo
How to Cite
Nguyễn, V. T. ., Nguyễn, T. H. ., Trần , T. H. T. ., Đào, N. B. ., Đoàn , T. K. P. ., Lương , T. L. A. ., Phạm , Đình M. ., & Vũ , T. H. . (2023). THE COMPLETION OF FLUORESCENCE IN SITU HYBRIDIZATION (FISH) IN DETECTION OF CHROMOSOMAL ANEUPLOIDY IN HUMAN SPERM. Vietnam Medical Journal, 524(1A). https://doi.org/10.51298/vmj.v524i1A.4644

THE COMPLETION OF FLUORESCENCE IN SITU HYBRIDIZATION (FISH) IN DETECTION OF CHROMOSOMAL ANEUPLOIDY IN HUMAN SPERM

Việt Trung Nguyễn1, Thị Huyền Nguyễn2, Thị Huyền Trang Trần 1, Ngọc Bắc Đào1, Thị Kim Phượng Đoàn 1, Thị Lan Anh Lương 1, Đình Minh Phạm 2, Thị Hà Vũ 1,
1 Hanoi medical university
2 Gentis Genetic Analysis Services Joint Stock Company

Main Article Content

Abstract

Sperm aneuploidy can directly affect pregnancy outcomes. Actually, fluorescence in situ hybridization (FISH) is the most popular and dominant method of determining aneuploidy in sperm cells. Objective of our study was to complete the in situ fluorescence hybridization technique to analyze aneuploidy from sperm cells. Methods: 15 semen samples were conducted to FISH for analysis aneuploidy rate at the Center for Clinical Genetics and Genomics, Hanoi Medical University Hospital, and Gentis International testing centre, Gentis Genetic Analysis Services Joint Stock Company. Results: 15/15 sperm samples successfully performed in FISH to analyze sperm aneuploidy for five chromosomes (13, 18, 21, X, Y). Conclusion: The FISH technique has several stages, each with its own characteristics consistent with the structure, membrane properties and DNA of the sperm cell, to ensure a successful procedure and clear analytical results, allowing accurate detection of chromosomal aberrations from sperm cells.

Article Details

Keywords

Fluorescence in situ hybridization, chromosomes of sperm cell, chromosomal aneuploidy

References

1. Hwang K, Weedin JW, Lamb DJ. The use of fluorescent in situ hybridization in male infertility. Ther Adv Urol. 2010;2(4):157-169. doi:10.1177/ 1756287210373758
2. Al-Hassan S, Hellani A, Al-Shahrani A, Al-Deery M, Jaroudi K, Coskun S. Sperm chromosomal abnormalities in patients with unexplained recurrent abortions. Arch Androl. 2005;51(1):69-76. doi:10.1080/014850190518062
3. Zidi-Jrah I, Hajlaoui A, Mougou-Zerelli S, et al. Relationship between sperm aneuploidy, sperm DNA integrity, chromatin packaging, traditional semen parameters, and recurrent pregnancy loss. Fertil Steril. 2016;105(1):58-64. doi:10.1016/j.fertnstert.2015.09.041
4. Munné S, Alikani M, Tomkin G, Grifo J, Cohen J. Embryo morphology, developmental rates, and maternal age are correlated with chromosome abnormalities. Fertil Steril. 1995;64(2):382-391.
5. Male infertility: establishing sperm aneuploidy thresholds in the laboratory - PMC. Accessed November 6, 2022. https:// www.ncbi.nlm.nih.gov/pmc/articles/PMC6439035/
6. Sperm aneuploidy: when to stop counting? - Fertility and Sterility. Accessed November 6, 2022. https://www.fertstert.org/article/S0015-0282(09)02795-2/fulltext
7. The Art of Packaging the Sperm Genome: Molecular and Structural Basis of the Histone-To-Protamine Exchange - PMC. Accessed November 6, 2022. https:// www.ncbi.nlm.nih.gov/pmc/articles/PMC9258737/
8. Akmal M, Aulanni’am A, Widodo MA, Sumitro SB, Purnomo BB, Widodo. The important role of protamine in spermatogenesis and quality of sperm: A mini review. Asian Pacific Journal of Reproduction. 2016;5(5):357-360. doi:10.1016/j.apjr.2016.07.013
9. Thioredoxin-dependent disulfide bond reduction is required for protamine eviction from sperm chromatin - PubMed. Accessed November 6, 2022. https:// pubmed.ncbi.nlm.nih.gov/28031247/