Article Sidebar
Views PDF: 278
LINEAGES DISTRIBUTION OF MYCOBACTRERIUM TUBERCULOSIS IN DONG THAP PROVINCE BY USING NEXT GENERATION SEQUENCING (NGS) TECHNOLOGY
Main Article Content
Abstract
Background: Tuberculosis is still a major puplic health concern today. In the Mekong Delta River, the Incedence of new TB and drug-resistant TB in some province, like Đong Thap, has not decreased much, creating a burden of disease, seriously affecting the region’s socio-economics. To support a strategy for sustainable TB reduction, molecular epidemiological studies of TB bacteria should be performed. Objectives: To determine the distributing proportion and genetic characteristics of Mycobacterium tuberculosis lineages in Dong Thap province. Materials and methods: The Mycobacterium tuberculosis samples collected from patients was diagnosed with all kinds of tuberculosis at the tuberculosis group in district, town, city and hospital of Dong Thap province. The collected samples were isolated and bacterial DNA was extracted, whole genome sequenced and data were analysed using bioinformatic tools. Results: Among 195 samples of tuberculosis strains analyzed, the rate of Bejing strain accounted for 46.1%, EAI strain accounted for 47.2% and T/H strain accounted for 6.7%. Among the tuberculosis strains detected, in Bejing linage, the sub-strain Bejing-RD181 accounted for the highest (67.8%). In EAI linage, the substrain EAI4-VNM accounted for the highest (76%) and in the T/H linage, the substrain T/H accounted for 38.5% and T1/T2/T3/T5 accounted for 30.8%. The other substrains account for a low percentage. The Bejing strain was common among patients with drug-resistant TB (85.4%). The community-based TB patients (55.2%) and recurrent TB (72.7%) belonged to the EAI lineage mainly. In the group of patients with severe TB at the hospital, TB strains were evenly distributed in all 3 strains: Bejing (46.3%), EAI (41.5%) and T/H (12.2%). Conclusions: The findings of the study has showed that the new tuberculosis strain of Dong Thap province belong to the Bejing and EAI strains predominate.
Article Details
Keywords
Mycobacterium tuberculosis, Dong Thap, next generation sequencing
References
2. Brosch R., Gordon S.V., Marmiesse M., et al. (2002), "A new evolutionary scenario for the Mycobacterium tuberculosis complex." Proc Nalt Acad Sci USA 99: 3684-9.
3. Nguyễn Thị Vân Anh (2014), “Dịch tễ học phân tử bệnh lao - ứng dụng và thành tựu”. Tạp chí Y học dự phòng, tập XXIV, số 6 (155).
4. Đỗ Tấn Khang, Trần Thị Thanh Khương , Nguyễn Phạm Anh Thi và Trần Thị Mỹ Duyên (2019), “Tiềm năng mở rộng ứng dụng công nghệ giải trình tự gen thế hệ mới ở đồng bằng sông Cửu Long”. Tạp chí Khoa học Trường Đại học Cần Thơ, 55, Số chuyên đề: Công nghệ Sinh học (2019)(1): 1-13.
5. Coll, F., McNerney, R., Guerra-Assunção, J. A., Glynn, J. R., Perdigão, J., Viveiros, M., Portugal, I., Pain, A., Martin, N., & Clark, T. G. (2014), “A robust SNP barcode for typing Mycobacterium tuberculosis complex strains. Nature communications, 5, 4812.
6. Phelan, J. E., O'Sullivan, D. M., Machado, D., Ramos, J., Oppong, Y. E. A., Campino, S., O'Grady, J., McNerney, R., Hibberd, M. L., Viveiros, M., Huggett, J. F., & Clark, T. G. (2019), “Integrating informatics tools and portable sequencing technology for rapid detection of resistance to anti-tuberculous drugs”. Genome medicine, 11(1), 41.
7. Nguyễn Thị Vân Anh (2009), “Dịch tễ học phân tử bệnh lao Tại Việt Nam (2003- 2009)" Luận án tóm tắt, Viện vệ sinh dịch tễ Hà Nội.
8. Nguyễn Văn Hưng và cs. (2011), “Sự phân bố của Mycobacterium tuberculosis kiểu gen Beijing và mối liên quan đến tính kháng thuốc lao tại Việt Nam”. Journal Franco-Vietnamien de pneumologie 2011; 02(02) 1 - 86.