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Date Published: 24/03/2023
Abstract Views: 640
Views PDF: 280
DOI: https://doi.org/10.51298/vmj.v524i1B.4796
Issue
Vol. 524 No. 1B (2023)
Section
Các bài báo
How to Cite
Nguyễn, H. L. T. ., Phạm , N. L. ., Trương , M. N. ., Lê, M. T. ., Lê, M. T. ., Thái , H. H. ., & Trương , V. Đạt . (2023). STIMULTANEOUS DETERMINATION OF NARINGIN AND HESPERIDIN DEVELOPMENT IN YOUNG POMELO BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY METHOD – PDA DETECTOR. Vietnam Medical Journal, 524(1B). https://doi.org/10.51298/vmj.v524i1B.4796

STIMULTANEOUS DETERMINATION OF NARINGIN AND HESPERIDIN DEVELOPMENT IN YOUNG POMELO BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY METHOD – PDA DETECTOR

Hữu Lạc Thủy Nguyễn1, Ngọc Liên Phạm 1, Minh Nhựt Trương 1, Minh Trí Lê1, Minh Tài Lê, Hồng Hạnh Thái , Văn Đạt Trương 1,
1 University of Medicine and Pharmacy at Ho Chi Minh City

Main Article Content

Abstract

Objectives: To develop an analytical methodology to simultaneously determine naringin and hesperidin in young pomelo powder. Subjects and methods: Naringin and hesperidin in young pomelo powder were determined by HPLC method. Results: The suitable chromatographic conditions for separation of naringin and hesperidin were obtained using C18 column; mobile phase: acetonitrile – acetic acid 0.1 % (20 : 80); flow rate 1 ml/min; injection volume 10 μl; column temperature 40 oC; 283 nm PDA detector. The process was validated the system suitsability; selectivity; linearity range naringin: 0.3 – 250.0 (ppm), y = 16951.7x; r = 0.9999 and hesperidin: 0.3 – 25.0 (ppm), y = 20736.9x; r = 1.0000; precision of both compounds with RSD < 2.0%; and accuracy with recovery ratio in 90 – 107 (%). Conclusion: The process could be applied to determine the content of marker in young pomelo powder.

Article Details

Keywords

naringin, hesperidin, young pomelo powder, HPLC.

References

1. AOAC International. AOAC Guidelines for Single Laboratory Validation of Chemical Methods for Dietary Supplements and Botanicals. Section 3.4. 2002:17-22.
2. Ani PN, Abel HC. Nutrient, phytochemical, and antinutrient composition of Citrus maxima fruit juice and peel extract. Food Sci Nutr. 2018;6(3):653-658.
3. Đỗ Tất Lợi. Những cây thuốc và vị thuốc Việt Nam. Nhà xuất bản Y học. 2006.
4. ICH Harmonised Tripartite Guideline. Validation of analytical procedures: text and methodology 2005:1-13.
5. Moiseev DV, Buzuk GN, Shelyuto VL. Identification of flavonoids in plants by HPLC. Pharm Chem J. 2011;45:47-50.
6. Tocmo R, Pena-Fronteras J, Calumba KF, Mendoza M, Johnson JJ. Valorization of pomelo (Citrus grandis Osbeck) peel: A review of current utilization, phytochemistry, bioactivities, and mechanisms of action. Compr Rev Food 2020;19(4):1969-2012.
7. Trịnh Thị Điệp, Trần Minh Ngọc, Nguyễn Tuấn Anh và cs. Xây dựng phương pháp định tính, định lượng đồng thời naringin và hesperidin trong vỏ quả các loài thuộc chi Citrus bằng sắc ký lỏng hiệu năng cao. Tạp chí Dược liệu. 2011;16(1+2):82-87.
8. Yu EA, Kim GS, Lee JE, et al. Flavonoid profiles of immature and mature fruit tissues of Citrus grandis Osbeck (Dangyuja) and overall contribution to the antioxidant effect. Biomed Chromatogr. 2015;29(7):1131.