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Date Published: 30/03/2023
Abstract Views: 778
Views PDF: 423
DOI: https://doi.org/10.51298/vmj.v524i2.4820
Issue
Vol. 524 No. 2 (2023)
Section
Các bài báo
How to Cite
Nguyễn, T. T. ., Nguyễn, H. N. ., Nguyễn, A. T. ., Nguyễn, Đăng T. ., & Ngô, K. Đức . (2023). IN VITRO ANTIOXIDANT AND ANTI-INFLAMMATORY ACTIVITY OF FLOWER EXTRACT OF CLITORIA TERNATEA L. USING DPPH METHOD AND PROTEIN DENATURATION ASSAY. Vietnam Medical Journal, 524(2). https://doi.org/10.51298/vmj.v524i2.4820

IN VITRO ANTIOXIDANT AND ANTI-INFLAMMATORY ACTIVITY OF FLOWER EXTRACT OF CLITORIA TERNATEA L. USING DPPH METHOD AND PROTEIN DENATURATION ASSAY

Thanh Tuyền Nguyễn1,, Hàn Ny Nguyễn1, Anh Tuấn Nguyễn1, Đăng Tiến Nguyễn1, Kiến Đức Ngô1
1 University of Medicine and Pharmacy at Ho Chi Minh City

Main Article Content

Abstract

Clitoria ternatea L. flower contains anthocyanins known as promising sources of bioactive compounds since they have been traditionally used for the treatment of various diseases. The study was for evaluation of in vitro antioxidant and anti-inflammatory activity of total ethanol extract and the fractions of Clitoria ternatea L. The results showed that total ethanol extract and all of the fractions of chloroform, ethyl acetate, buthanol, water had in vitro antioxidant and anti-inflammatory activity. In which, ethyl acetat extract had the highest effect with IC50 of 19,51 ± 1,14 µg/mL in the antioxidant test (higher than that of acid ascorbic 7,98 ± 0,46 µg/mL) and IC50 of 3,02 ± 0,09 mg/mL in the anti-inflammatory test (higher than that of diclofenac natri 1,28 ± 0,02 mg/mL).

Article Details

Keywords

Clitoria ternatea L., antioxidant, anti-inflammatory.

References

1. Võ Văn Chi. Từ điển cây thuốc Việt Nam. Tập 1. Nhà xuất bản Y học, Hà Nội. 2012:883-885.
2. Nguyễn Hồng Nhung. Nghiên cứu thành phần hóa học hướng tác dụng chống oxy hóa của lá và thân cây Đậu biếc. Luận văn Thạc sĩ Dược học. Đại học Y Dược TP. Hồ Chí Minh. 2020.
3. Mahmad N, Taha RM, Othman R, et al. Anthocyanin as potential source for antimicrobial activity in Clitoria ternatea L. and Dioscorea alata L. Pigment and Resin Technology. 2018; 47(6): 490-495.
4. Kumar SB, Bhat KI. In-vitro cytotoxic activity studies of Clitoria ternatea Linn flower extracts. International Journal of Pharmaceutical Sciences Review and Research. 2011;6(2):120-121.
5. Rajamanickam M, Kalaivanan P, Sivagnanam I. Evaluation of anti-oxidant and anti-diabetic activity of flower extract of Clitoria ternatea L. Journal of Applied Pharmaceutical Science. 2015; 5(8):131-138.
6. Yesmin S, Paul A, Naz T, et al. Membrane stabilization as a mechanism of the anti-inflammatory activity of ethanol root extract of Choi (Piper chaba). Clinical Phytoscience. 2020; 6(1):1-10.
7. Kim SM, Lim ST. Enhanced antioxidant activity of rice bran extract by carbohydrase treatment. Journal of Cereal Science. 2016;68:116-121.
8. Nair V, Bang WY, Schreckinger E, et al. Protective role of ternatin anthocyanins and quercetin glycosides from Butterfly pea (Clitoria ternatea Leguminosae) blue flower petals against lipopolysaccharide (LPS)-induced inflammation in macrophage cells. Journal of Agricultural and Food Chemistry. 2015;63(28):6355-6365.