MỐI LIÊN QUAN GIỮA ĐỨT GÃY DNA CỦA TINH TRÙNG VÀ HÌNH THÁI TINH TRÙNG

Abstract

Objective: To evalate the relationship between the degree of DNA damage sperm (DFI- DNA fragmentation index) with sperm morphology of 90 infertile couples. Subjects and research methods: infuture cohort study of 90 infertile couples at Reproductive Support Center of Ha Noi Medical University Hospital from August 2020 - December 2021. The husband with fresh ejaculate sperm was tested Semen analysic WHO 2010 and Halosperm test, after a period of ebstinence 3-5 days, divided into 3study groups. Group N1: 21 peoples with normal morphology sperm, group N2: 59 peoples with sperm head malformation (small head, micocephatic, triangular head, less crosome...), groupN3: 10 peoples with other malformed sperm (deviated neck, curved tail, short tail, two tails...). Results: The mean DFI rate of N2 group was highest, the fewest subjects with DFI < 15%, more subjects with DFI > 30% compare with N3 group and N1 group were statistically significant. DFI is negatively correlate with density and motility of sperm. DFI ROC curve for  the inferility dianogis was significant when compare with density and motility, cutt off Halosperm respectively 20.2%; 15.4% and AUC (area under curve)  0.785; 0.639 with p<0.05. Conclusion: DFI has a statisticlly significant corretion with N2, no correlation between DFI with N3,N1, p>0,05. The mean DFI rate of N2 group was highest compare N3,N1. Distribution of DFI in N2 group, also significantly different from N1, N3 group, p<0,05. Unbroken (DFI< 15%) accounts for the lowest percentage and many subjects with DFI ≥ 30% accounted for a high. DFI is negatively correlated with density and motility of sperm. Threshold cutt off 20.2%; 15.4% to diagnose infertility due to Oligo, Weak sperm.