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Date Published: 30/03/2023
Abstract Views: 175
Views PDF: 119
DOI: https://doi.org/10.51298/vmj.v524i2.4868
Issue
Vol. 524 No. 2 (2023)
Section
Các bài báo
How to Cite
Nguyễn, T. A. ., Bùi , T. T. ., Lê, T. T. T. ., & Nguyễn, T. D. . (2023). INITIALLY EVALUATION OF A REAL-TIME PCR FOR DETECTION OF H. PYLORI ISOLATED FROM THE GASTROENDOSCOPY SAMPLES. Vietnam Medical Journal, 524(2). https://doi.org/10.51298/vmj.v524i2.4868

INITIALLY EVALUATION OF A REAL-TIME PCR FOR DETECTION OF H. PYLORI ISOLATED FROM THE GASTROENDOSCOPY SAMPLES

Thùy An Nguyễn1,2,, Thế Trung Bùi 2, Thị Thanh Thùy Lê2, Tiến Dũng Nguyễn3
1 VNUHCM-University Of Science
2 THE CHILDREN’S HOSPITAL 2
3 National Agro-forestry-Fisheries Quality Assurance Department-Branch 4

Main Article Content

Abstract

Objectives: Design and initially evaluation of a real-time PCR for detection of H. pylori isolated from the clinical samples based on 23S rRNA gene with primers CRF-4 and CRR-1(6). Methods: The specificity of real-time PCR method was evaluated by on: (1) twenty-five strains of non-H. pylori and fungi found in gastroendoscopy samples and (2) one hundred and fifty cultured strains from pediatric gastric antrum samples, which included strains identified as H. pylori and other suspected strains. The coverage of amplified gene fragments was confirmed by sequencing of the 135 bp amplicons from nine strains with differences in staining morphology, colony characteristics and identified biochemical data. Results: The specificity of the PCR assay was confirmed by negative results for microbiota on endoscopy pathway and positive for 150 H. pylori isolated strains. The specificity of this assay was measured by comparing the amplified sequence with 10 strains of Helicobacter species. Results showed that the amplified target gene sequences were similar (97.78% - 99.26%) to the H. pylori sequence retrieved from GenBank. Conclusion: The real-time PCR assay based on the 23S rRNA gene with primers CRF-4 và CRR-1 for H. pylori detection was successfully established with specificity being 100%.

Article Details

Keywords

real-time PCR, 23S rRNA, detection, Helicobacter pylori.

References

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