ĐẶC ĐIỂM HÌNH THÁI TINH TRÙNG ĐƯỢC NHUỘM BẰNG PAPANICOLAOU, GIEMSA VÀ EOSIN

Abstract

Objectives: Comparison of Morphological sperm stained by Papanicolaou, Giemsa, Eosin methods. Material and methods: A descriptive cross-sectional study of 30 semen samples with normal limited parameters. Each sample stained by Papanicolaou, Giemsa, Eosin methods. The slides were observed under the light microscope, the spermatozoa images were captured and measured in size using Infinity Analyze software. Results: The normal and abnormal spermatozoa morphology recorded by the 3 methods had no statistically significant differences. Sperm head size stained by Eosin was larger than sperm head size in Papanicolaou, Giemsa methods. The rate of sperm head length/ width, and length of the tail were not different between the methods. The head and acrosome were very clearly observed in Papanicolaou, Giemsa. The tail and middle piece were very clearly observed in Papanicolaou. Conclusion: Papanicolaou is a suitable method in the study of morphological sperm. Giemsa and Eosin staining can be applied in routine semen testing.