COMPARISON OF THE MICROSCAN WALKAWAY MACHINE AND API KIT OF THE IDENTIFICATION IN PATIENTS WITH GASTROINTESTINAL AND URINARY TRACT INFECTIONS AT CITY CHILDREN'S HOSPITAL

Thị Thanh Mai Nguyễn1,, Phúc Mỹ Võ 2, Minh Hiển Võ 2
1 Hong Bang university
2 THE CHILDREN’S HOSPITAL

Main Article Content

Abstract

Background: Accuracy and quick identification of bacterial pathogens causing gastrointestinal and urinary tract diseases are essential factors in determining the infectious etiology for Pediatric disease. Objective: Study to compare the correlation of identification results between the MicroScan WalkAway automatic identification machine and the API rapid kit (bioMérieux) on pediatric patients with gastrointestinal and urinary tract infections. Methods: Cross-sectional description - From 04/2022 - 08/2022 at Department of Microbiology - City Children's Hospital. Results: The study collected 56 strains, of which 12 strains of Gram-negative bacilli were isolated from urine samples, 46 Gram-negative strains were isolated from stool samples and 5 strains ATCC . Of these 91.8% were correctly identified at the cultivar and species level using both systems. Identical concordance in 12 urine samples was 83.3%, stool samples was 93.3%. Average time to get test results is 12.5 hours for MicroScan and 24 hours for Api kit. Conclusion: A method to replace the MicroScan WalkAway automatic analyzer microbial identification technique with an API rapid (bioMérieux) for all types of specimens if the system has problems or problems during operation.

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References

1. O'Hara CM, Tenover FC, Miller JM. Parallel comparison of accuracy of API 20E, Vitek GNI, MicroScan Walk/Away Rapid ID, and Becton Dickinson Cobas Micro ID-E/NF for identification of members of the family Enterobacteriaceae and common gram-negative, non-glucose-fermenting bacilli. J Clin Microbiol. 1993 Dec;31(12):3165-9.
2. McGregor A, Schio F, Beaton S, Boulton V, Perman M, Gilbert G. The MicroScan WalkAway diagnostic microbiology system--an evaluation. Pathology. 1995 Apr;27(2):172-6.
3. Fader RC, Weaver E, Fossett R, Toyras M, Vanderlaan J, Gibbs D, Wang A, Thierjung N. Multilaboratory study of the Biomic automated well-reading instrument versus MicroScan WalkAway for reading MicroScan antimicrobial susceptibility and identification panels. J Clin Microbiol. 2013 May;51(5):1548
4. Hernández-Durán, Melissa, et al. "Comparison of the microscan walkaway and Vitek 2 compact systems for the identification and susceptibility of clinical gram-positive and gram-negative bacteria." Investigación en discapacidad 6.3 (2017): 105-114.
5. Snyder JW, Munier GK, Johnson CL. Direct comparison of the BD phoenix system with the MicroScan WalkAway system for identification and antimicrobial susceptibility testing of Enterobacteriaceae and nonfermentative gram-negative organisms. J Clin Microbiol. 2008 Jul;46(7):2327-33.
6. Jin WY, Jang SJ, Lee MJ, Park G, Kim MJ, Kook JK, Kim DM, Moon DS, Park YJ. Evaluation of VITEK 2, MicroScan, and Phoenix for identification of clinical isolates and reference strains. Diagn Microbiol Infect Dis. 2011 Aug;70(4):442-7.
7. Holmes B, Willcox WR, Lapage SP. Identification of Enterobacteriaceae by the API 20E system. J Clin Pathol. 1978 Jan;31(1):22-30
8. Aldridge KE, Hodges RL. Correlation studies of entero-set 20, API 20E and conventional media systems for Enterobacteriaceae identification. J Clin Microbiol. 1981 Jan;13(1):120-5.
9. Darbandi, F. (2011). Parallel Comparison of Accuracy in Vitek2 Auto analyzer and API 20 E/API 20 NE Microsystems.