XÁC NHẬN GIÁ TRỊ SỬ DỤNG PHƯƠNG PHÁP GỘP QUE TRONG KỸ THUẬT REAL-TIME RT PCR CHẨN ĐOÁN SARS-COV-2

Abstract

Background: The swab pooling method in real-time RT-PCR diagnostics for SARS-CoV-2 is commonly used due to saving time and resources for laboratory, quickly screening while ensuring reliability. Laboratories need to perform verification of swab pooling before putting them into use. Objective: Comparison of the ability of real-time RT PCR to detect SARS-CoV-2 on a pooled samples compared to a single sample. Materials and method: Descriptive and experimental study. The 2-Pooled, 5-pooled and 10-pooled were created from nasopharyngeal swab samples that were confirmed negative and positive for SARS-CoV-2 by real-time RT PCR on Allplex™ SARS-CoV-2 Assay (Seegene) and Alinity m SARS-CoV-2 assay (Abbott). Results: The percentage of positive agreement of SARS-CoV-2 in 2-Pooled, 5-pooled and 10-pooled were 100% when compared with the single sample with the mean Ct difference not exceeding 1.7. Conclusion: The study showed that the ability to detect samples positive for SARS-CoV-2 by real-time RT PCR in the pooled sample was equivalent to the single sample.