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CHROMOSOME ABNORMAL BLASTOCYST RATE BY AGE GROUPS OF INFERTILE PATIENTS UNDERGOING IN VITRO FERTILIZATION TREATMENT
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Abstract
Objective: To evaluate embryonic chromosomal abnormalities by age groups of infertile patients with indications for in vitro fertilization treatment combined with preimplantation genetic testing for aneuploidy. Subjects and methods: prospective description of 661 blastocysts from 186 infertile patients with indications for in vitro fertilization combined with pre-implantation genetic testing for aneuploidies/PGT-A. Results: The proportion of normal chromosomal embryos was equal between group I (under 30 years old) and group II (30-35 years old), 51.1 and 54.1%, respectively (P(1-2)=0,613); The lowest rate has shown in the group III (>35 years old) with the rate of 35.7%, P(1-3); P(2-3) <0,05. There was no statistically significant difference in abnormal chromosomal blastocysts rate between group I and group II (P(1-2)=0,310) but increased significantly in group III at 47.2% (P(1-3); P(2-3)<0,001). The rate of chromosomal numerical abnormalities increased with advanced maternal age (p<0.05). In contrast, the rate of structural abnormalities shows the opposite trend, although the difference was insignificant (p=0.148). The rate of mosaic embryos in the three groups was 25%, 16.5%, and 17.1%, respectively (P(1-2)=0,069; P(1-3)=0,101; P(2-3)=0,843). Among the chromosomal numerical abnormality, the ratio of monosomy and trisomy was similar in the three groups (P(1-2);P(1-3); P(2-3)>0,05). Conclusion: In infertile patients with indications for IVF – PGT-A, the rate of abnormal chromosomal blastocyst increased statistically significantly when maternal age was over 35. The numerical chromosomal abnormality increased statistically with the advanced maternal age. Still, there was no significant difference in the rate of the structural chromosomal abnormal blastocyst, the ratio of the mosaic embryo, the rate of monosomy, and trisomy embryos between the study groups.
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Keywords
Abnormal chromosome, RIF (recurrent implantation failure), RPL (recurrent pregnancy loss), advanced maternal age.
References
2. Liu X-Y, Fan Q, Wang J, et al. Higher chromosomal abnormality rate in blastocysts from young patients with idiopathic recurrent pregnancy loss. Fertility and Sterility. 2020;113(4):853-864.
3. Bashiri A, Halper KI, Orvieto R. Recurrent Implantation Failure-update overview on etiology, diagnosis, treatment and future directions. Reprod Biol Endocrinol. 2018; 16(1):121.
4. Barbakadze L, Kristesashvili J, Khonelidze N, Tsagareishvili G. The correlations of anti-mullerian hormone, follicle-stimulating hormone and antral follicle count in different age groups of infertile women. Int J Fertil Steril. 2015;8(4):393-398.
5. Kozlowski IF, Carneiro MC, Rosa VBD, Schuffner A. Correlation between anti-Müllerian hormone, age, and number of oocytes: A retrospective study in a Brazilian in vitro fertilization center. JBRA Assist Reprod. 2022;26(2):214-221.
6. Munné S, Held KR, Magli CM, et al. Intra-age, intercenter, and intercycle differences in chromosome abnormalities in oocytes. Fertil Steril. 2012;97(4):935-942.
7. Cimadomo D, Fabozzi G, Vaiarelli A, Ubaldi N, Ubaldi FM, Rienzi L. Impact of Maternal Age on Oocyte and Embryo Competence. Front Endocrinol (Lausanne). 2018;9:327.
8. Sainte-Rose R, Petit C, Dijols L, Frapsauce C, Guerif F. Extended embryo culture is effective for patients of an advanced maternal age. Sci Rep. 2021;11(1):13499.
9. Yeoh MH, Chen JJ, Sinthamoney E, Wong PS. Clinical outcome: the relationship between mosaicism and advanced maternal age with the use of Next Generation Sequencing (NGS). Reproductive BioMedicine Online. 2019;38:e48.
10. Gui J, Ding J, Yin T, Liu Q, Xie Q, Ming L. Chromosomal analysis of 262 miscarried conceptuses: a retrospective study. BMC Pregnancy Childbirth. 2022;22(1):906.