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Date Published: 27/04/2023
Abstract Views: 834
Views PDF: 348
DOI: https://doi.org/10.51298/vmj.v525i2.5176
Issue
Vol. 525 No. 2 (2023)
Section
Các bài báo
How to Cite
Nguyễn, H. B. ., Nguyễn, H. H. ., Mai , T. B. C. ., Lưu , N. T. T. ., Lê, M. K. ., & Nguyễn, T. B. S. . (2023). SURVEY OF SUBCLINICAL FEATURES AND FREQUENCY OF EGFR, KRAS MUTATIONS IN PATIENTS WITH NON-SMALL CELL LUNG CANCER. Vietnam Medical Journal, 525(2). https://doi.org/10.51298/vmj.v525i2.5176

SURVEY OF SUBCLINICAL FEATURES AND FREQUENCY OF EGFR, KRAS MUTATIONS IN PATIENTS WITH NON-SMALL CELL LUNG CANCER

Hoàng Bắc Nguyễn1,2, Hữu Huy Nguyễn1, Thị Bích Chi Mai 1, Nguyễn Trung Thông Lưu 1, Minh Khôi Lê1,2, Thị Băng Sương Nguyễn1,2,
1 University of Medicine and Pharmacy at Ho Chi Minh City hospital
2 University of Medicine and Pharmacy at Ho Chi Minh City

Main Article Content

Abstract

Introduction: Non-small cell lung cancer (NSCLC) is a cancer with increasing morbidity and mortality in Vietnam. Molecular assays of EGFR, KRAS are widely used to guide individualized treatment in NSCLC patients. Objective: To investigate subclinical features and frequency of EGFR, KRAS mutations in patients with non-small cell lung cancer in University medical center HCMC. Materials and Methods: DNA was isolated from 111 FFPE samples collected from NSCLC patients. DNA libraries were sequenced on NextSeq instrument (Illumina). Results:  According to NGS results, mutations were detected in EGFR (52/111, 46,8% of patients), KRAS (16/111, 14,4%). EGFR mutations were frequent in women, <60 years old, adenocarcinoma. EGFR mutations were frequent in women, <60 years old, adenocarcinoma. Meanwhile, KRAS mutations were frequent in men, ≥ 60 years old, adenocarcinoma. Conclusions: NGS allows for specific detection and precise identification of gene mutations in NSCLC patients.

Article Details

Keywords

Non-small cell lung cancer, Next-generation sequencing

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