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ASSESSMENT OF THE ISOLATION, PROLIFERATION AND MIGRATION ABILITY OF FIBROBLASTS DERIVED FROM PATIENTS WITH CHRONIC WOUND
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Abstract
Objective: To evaluate the morphology, isolation, proliferation and migration ability of cultured fibroblasts derived from patients with chronic wounds. Subjects and methods: Prospective study on 60 dermal samples from 3 distinct locations of 20 patients with chronic pressure ulcers and diabetic ulcers. Skin samples were cultured according to the Freshney RI 2003 procedure to assess fibroblast growth time, generation time of isolation, proliferation and migration rates between different sites of the two groups and comparison with normal dermal fibroblasts. Results: The dermal samples at 3 different locations all grew fibroblasts. However, the wound base fibroblasts (position 1) showed aging, did not retain its phenotype and died floating on the surface of the culture plate, could not be isolated to the P4 generation. Fibroblasts from the wound edge (position 2) and the healing skin next to the wound (position 3) could be isolated to the P3, P4, P5 generation and did not change morphology. Fibroblasts from chronic wound at positions 2, 3 have slower proliferation rate, experimental wound healing migration when compared with normal dermal fibroblasts. Conclusions: Fibroblasts at the wound edges and in healing skin adjacent to the wound from chronic ulcers can be established in culture without changing morphology. However, the proliferation rate and migratory capacity of fibroblasts deriving from chronic wound was slower than that of normal dermal fibroblasts.
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Keywords
Fibroblasts, chronic wound
References
2. Mendez, M.V., et al., The proliferative capacity of neonatal skin fibroblasts is reduced after exposure to venous ulcer wound fluid: a potential mechanism for senescence in venous ulcers. Journal of vascular surgery, 1999. 30(4): p. 734-743.
3. Brem, H., et al., Primary cultured fibroblasts derived from patients with chronic wounds: a methodology to produce human cell lines and test putative growth factor therapy such as GMCSF. Journal of translational medicine, 2008. 6(1): p. 1-9.
4. Pansani, T.N., et al., Effects of low-level laser therapy on the proliferation and apoptosis of gingival fibroblasts treated with zoledronic acid. 2014. 43(8): p. 1030-1034.
5. Houreld, N., H.J.P. Abrahamse, and l. surgery, In vitro exposure of wounded diabetic fibroblast cells to a helium-neon laser at 5 and 16 J/cm2. 2007. 25(2): p. 78-84.
6. Loots, M.A., et al., Cultured fibroblasts from chronic diabetic wounds on the lower extremity (non-insulin-dependent diabetes mellitus) show disturbed proliferation. 1999. 291(2): p. 93-99.
7. Wall, I.B., et al., Fibroblast dysfunction is a key factor in the non-healing of chronic venous leg ulcers. 2008. 128(10): p. 2526-2540.
8. Vande Berg, J.S., et al., Cultured pressure ulcer fibroblasts show replicative senescence with elevated production of plasmin, plasminogen activator inhibitor‐1, and transforming growth factor‐β1. Wound repair and regeneration, 2005. 13(1): p. 76-83.