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Date Published: 27/04/2023
Abstract Views: 295
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DOI: https://doi.org/10.51298/vmj.v525i2.5233
Issue
Vol. 525 No. 2 (2023)
Section
Các bài báo
How to Cite
Nguyễn, T. B. P. ., Đinh , V. H. ., Nguyễn, N. T. ., Nguyễn, N. L. ., & Nguyễn, T. H. . (2023). IN VITRO ASSESSMENT OF THE EFFECT OF LOW LEVEL LASER THERAPY ON THE PROLIFERATION AND MIGRATION OF FIBROBLASTS DERIVED FROM PATIENTS WITH CHRONIC WOUNDS. Vietnam Medical Journal, 525(2). https://doi.org/10.51298/vmj.v525i2.5233

IN VITRO ASSESSMENT OF THE EFFECT OF LOW LEVEL LASER THERAPY ON THE PROLIFERATION AND MIGRATION OF FIBROBLASTS DERIVED FROM PATIENTS WITH CHRONIC WOUNDS

Thị Bích Phượng Nguyễn1,, Văn Hân Đinh 1, Ngọc Tuấn Nguyễn1, Như Lâm Nguyễn1, Thị Hương Nguyễn1
1 LE HUU TRAC NATIONAL BURNS HOSPITAL

Main Article Content

Abstract

Objectives: Evaluating the effects of low-level laser therapy on the proliferation and emigration of fibroblasts derived from chronic wound patients. Subjects and Methods: Dermal fibroblasts isolated from patients with pressure ulcers and diabetic ulcers according to the Freshney RI protocol (2003). The resulting fibroblasts will be subjected to LLLT irradiation with energy level 3J at wavelengths of 670, 780, 805, 980 nm with respectively irradiation time: 170, 72, 72 and 76s to evaluate proliferation and migration rate of fibroblasts between the laser group and the non-irradiated group. Results: When irradiating LLLT with 3J energy at all wavelengths, fibroblasts did not change morphology, increased more strongly than the non-irradiated group, the highest increase at two wavelengths 670 and 780 nm. The migration rate of fibroblasts was faster in the laser group than in the control group, which completely covered the culture plate at 3rd day. Conclusion: LLLT with 3J energy levels did not change the fibroblast phenotype; induce biostimulation, proliferation and migration of cultured fibroblast samples derived from patients with chronic wounds. However, the efficiency of LLLT is wavelength dependent, peaking at 670 and 780 nm.

Article Details

Keywords

Low-level laser therapy, fibroblasts, chronic wound

References

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