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Date Published: 01/06/2023
Abstract Views: 350
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DOI: https://doi.org/10.51298/vmj.v526i2.5500
Issue
Vol. 526 No. 2 (2023)
Section
Các bài báo
How to Cite
Nguyễn, T. B. ., & Phạm , M. A. . (2023). CHARACTERISTICS OF HLA GENOTYPE IN HEMATOPOIETIC STEM CELL DONORS AT VIETNAM NATIONAL CHILDREN’S HOSPITAL. Vietnam Medical Journal, 526(2). https://doi.org/10.51298/vmj.v526i2.5500

CHARACTERISTICS OF HLA GENOTYPE IN HEMATOPOIETIC STEM CELL DONORS AT VIETNAM NATIONAL CHILDREN’S HOSPITAL

Thanh Bình Nguyễn1,2,, Minh Anh Phạm 3
1 Hanoi medical university
2 Vietnam National Children's Hospital.
3 Viet Phap university

Main Article Content

Abstract

Background: HLA (Human Leukocyte Antigen) gene is located on chromosome 6’s short arm, devided in three classes and including several loci. The determination of HLA genotype is necessary in transplantation, especially in hematopoietic stem cell transplantation. Subjects and Methods: A cross-sectional study to determine the frequency of HLA-A, HLA-B, and HLA-DRB1 alleles of 138 hematopoietic stem cell dornors at Vietnam National Children’s Hospital from the year 2010 - 2020. The HLA genotyping was performed by PCR-SSP method using Micro SSPTM Generic HLA Class I and II DNA Typing Tray kit (One Lambda). Results: 14 alleles of HLA-A locus are determined in which the highest frequency alleles are A*11 (23.55%), A*02 (21.74%), A*33 (17.39%) and A*24 (13.04%). 26 allele of HLA-B locus are determined, in which the highest frequency alleles are B*15 (22,83%), B*58 (11,23%), B*46 (10,87%) and B*07 (7,61%). 9 allele of HLA-C locus are determined, in which the highest frequency alleles are C*03 (19,93%), C*07 (18,84%), C*01 (16,30%) and C*08 (15,22%). 14 allele of HLA-DRB1 are determined in which the highest frequency alleles are DRB1*12 (22,83%), DRB1*04 (11,23%), DRB1*03 (10,87%) and DRB1*15 (9,06%). There are 2176 haplotypes combined from 4 loci HLA-A, HLA-B, HLA-C, and HLA-DRB1, in which the highest frequency haplotypes are A*02/B*46/C*01/DRB1*09 (1.19%), A*33/B*58/C*03/DRB1*03 (0.97%), A*11/B*15/C*08/DRB1*12 (0.87%), and A*11/B*15/C*01/DRB1*12 (0.55%).

Article Details

Keywords

HLA allele, HLA haplotype, Hematopoietic Stem Cell Transplantation, Vietnam National Children’s Hospital

References

1. Montgomery, R.A et al. HLA in transplantation. Nat Rev Nephrol. 2018; (14): 558–570.
2. David G. Hernandez-Mej et al. Distributions of the HLA-A, HLA-B, HLA-C, HLA-DRB1, and HLA-DQB1 alleles and haplotype frequencies of 1763 stem cell donors in the Colombian Bone Marrow Registry typed by next- generation sequencing. Front Immunol. 2022; 13: 1057657
3. Hoa B.K. et al. HLA-A,-B,-C,-DRB1, and -DQB1 allels and haplotype in the Kinh population in Vietnam. Tissue Antigen. 2007; 71: 127-134.
4. Huy H.Q. et al. HLA-A and B phenotypes in Kinh people in Vietnam. Journal of Medical Research. 2000; 13(3): 24-31.
5. Que T.N. et al. Allele and Haplotype Frequencies of HLA-A, -B, -C, and DRB1 Genes in 3,750 Cord Blood Units From a Kinh Vietnamese Population. Front Immunol. 2022; doi: 10.3389/fimmu.2022.875283.
6. Gourraud et al. Hight-resolution HLA-A, HLA-B, HLA- DRB1 haplotype frequencies from the French Bone Marrow Donor Registry. American Society for Histocompatibility and Immunogenetics. 2015; 10: 1-4.
7. M.D. Do et al. High-Resolution HLA Typing of HLA-A, -B, -C, -DRB1, and -DQB1 in Kinh Vietnamese by Using Next-Generation Sequencing. Front Genet. 2020; 11:383.
8. Hajeer. et al. HLA-A, -B, -C, -DRB1 and -DQB1 allele and haplotype frequencies in Saudis using next generation sequencing technique. Tissue Antigens. 2013; 82(4): 252–258.
9. Pedron B. et al. Common genomic HLA haplotypes contributing to successful donor search in unrelated hematopoietic transplantation. Bone marrow transplantation. 2003; 31 (6): 423.