KIỂM CHỨNG MÔ HÌNH GÂY TIÊU CHẢY TRÊN CHUỘT NHẮT TRẮNG

Abstract

Objectives: In order to verify, re-evaluate the standards, research, and develop appropriate mice models of diarrhea models before conducting a survey on the effects of drugs, we chose the “kho than ta ha – KHTH” model according to the traditional medicine theory and the “antibiotic-associated diarrhea – AAD” model according to the modern medical theory to put into this research. Methods: In the two models, mice were divided into pathological control groups using rhubarb or senna extracts (the KHTH model), streptomycin and lincomycin (the AAD model) with different doses for 5 consecutive days, and the physiological group using distilled water. The image of mouse feces on blotting paper was used to determine the severity of diarrhea (normal, moderate, or severe). The model selection criteria included diarrhea rate (100%), mortality rate (<50%), and self-healing time (>48 hours). Results: In the KHTH model, the diarrhea time of mice was proportional to the dose of rhubarb or senna; however, there was no prolonged diarrhea, and 100% of the mice recovered after 24 hours of follow-up. In the AAD model, there was no similarity in antibiotic dose that caused the 100% diarrhea rate. The dose-adjusted oral antibiotic mice model (30 mg streptomycin + 40 mg lincomycin/10 g mouse) administered continuously for 3 days, followed by a 1% maintenance dose, met the criteria for selecting a diarrhea model. Conclusion: The AAD model in mice was verified and selected as follows: streptomycin 30 mg and lincomycin 40 mg/10 g mice for 3 days, maintaining 1% for 5 days.