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Date Published: 01/06/2023
Abstract Views: 327
Views PDF: 121
DOI: https://doi.org/10.51298/vmj.v526i2.5577
Issue
Vol. 526 No. 2 (2023)
Section
Các bài báo
How to Cite
Nguyễn, Q. T. ., & Nguyễn, D. T. . (2023). SOLUBILITY PREDICTION AND VECTOR DESIGN FOR THE EXPRESSION OF PROTEIN KOJA FROM THE BIOSYNTHETIC PATHWAY OF KOJIC ACID. Vietnam Medical Journal, 526(2). https://doi.org/10.51298/vmj.v526i2.5577

SOLUBILITY PREDICTION AND VECTOR DESIGN FOR THE EXPRESSION OF PROTEIN KOJA FROM THE BIOSYNTHETIC PATHWAY OF KOJIC ACID

Quốc Thái Nguyễn1,, Duy Thạch Nguyễn1
1 University of Medicine and Pharmacy at Ho Chi Minh City

Main Article Content

Abstract

Background: Kojic acid is a skin-whittening agent widely used in cosmetics. It is a secondary metabolite produced by a few Aspergillus species. Recent studies have identified that gene kojA played an important role in the biosynthetic pathway of kojic acid in Aspergillus oryzae. Objectives: This study aims to predict the solubility of protein KojA, and design the recombinant plasmid thereof to express KojA as soluble protein in Escherichia coli. Methods: A homology model of KojA was built using SWISS-MODEL server. Three tools including Protein-Sol, SOLart và SoDoPE were applied to predict the solubility of KojA and its fusion with tags. Results: All three tools predicted a poor solubility of KojA; the solubility can be improved with tags MBP and SUMO as suggested by SoDoPE. Conclusions: The study has predicted the solubility of protein KojA and proposed a recombinant plasmid in which kojA is fused with SUMO and condons optimized for expression in E. coli.       

Article Details

Keywords

acid kojic, gen kojA, khả năng hoà tan

References

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