SO SÁNH GIÁ TRỊ SỬ DỤNG PHƯƠNG PHÁP GỘP QUE VÀ GỘP DUNG DỊCH TRONG KỸ THUẬT REAL-TIME RT PCR CHẨN ĐOÁN SARS-COV-2

Abstract

Background: Swab pooling and media pooling methods in real-time RT-PCR diagnostics for SARS-CoV-2 is commonly used due to saving time and resources for laboratory, quickly screening while ensuring reliability. Laboratories need to perform verification of pooling methods before putting them into use. Objective: Comparison of the ability of real-time RT PCR to detect SARS-CoV-2 of swab pooling and media pooling methods. Materials and method: Descriptive and experimental study. The 2-Pooled, 5-pooled and 10-pooled were created from nasopharyngeal swab samples that were confirmed negative and positive for SARS-CoV-2 by real-time RT PCR on Allplex™ SARS-CoV-2 Assay (Seegene) and Alinity m SARS-CoV-2 Assay (Abbott). Results: The percentage of positive agreement of SARS-CoV-2 between the single sample and the pool samples (pool 2, pool 5 and pool 10) was 100% for the swab pooling method. With media pooling method, the percentage of positive agreement is 100% with samples with Ct range from 15 to 30, while at Ct range from 31 to 37, the percentage of positive agreement is 90%. Conclusion: The study showed that the percentage of positive agreement of SARS-CoV-2 and the mean Ct difference between the single sample and the pool samples in swab pooling method is better than that of media pooling method.