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Date Published: 24/07/2021
Abstract Views: 327
Views PDF: 156
DOI: https://doi.org/10.51298/vmj.v502i1.597
Issue
Vol. 502 No. 1 (2021)
Section
Các bài báo
How to Cite
Thị Hiệp Tuyết, N. ., Văn Trung, N. ., Thị Thái Thanh, N. ., Thị Hồng Nhạn, Đặng ., & Minh Tâm, L. . (2021). THE CORRELATION BETWEEN THE SPERM FRAGMENTATION DNA AND THE OUTCOME OF IN VITRO FERTILISATION. Vietnam Medical Journal, 502(1). https://doi.org/10.51298/vmj.v502i1.597

THE CORRELATION BETWEEN THE SPERM FRAGMENTATION DNA AND THE OUTCOME OF IN VITRO FERTILISATION

Nguyễn Thị Hiệp Tuyết, Nguyễn Văn Trung, Nguyễn Thị Thái Thanh, Đặng Thị Hồng Nhạn, Lê Minh Tâm

Main Article Content

Abstract

Objective: To evaluate the correlation between the sperm fragmentation DNA and the outcome of the intracytoplasmic sperm injection (ICSI) technique. Materials and methods: The descriptive cross-sectional study on 175 infertile couples by ICSI method at the Center for Reproductive Endocrinology and Infertility, Hue University Hospital. Evaluation of sperm DNA fragmentation by using sperm chromatin dispersion technique, the level of sperm DNA fragmentation is calculated by the value of the fragmentation index (DFI - DNA fragmentation index). After 16-18 hours of injection, the fertilized oocytes were determined by the presence of two pronuclei. Embryos were evaluated according to Istanbul consensus on the day 2 and 5. Results: The rate of fertilization in group DFI ≥ 30% was difference statistically lower than in DFI group <30% (80.27 ± 17.47% vs. 74.48 ± 17.32%, p = 0.046). There was no difference statistically between the two groups DFI for the quality of embryos on day 2, and day 5. Sperm DNA fragmentation had a correlation with the fertilization rate (r = -0.185, p = 0.014), linear regression equation: y =- 0.187x + 83.55. Conclusion:  Sperm DNA fragmentation has a negative correlation with post-ICSI fertilization rates. Sperm DNA fragmentation assay has a valuation in predict the results of infertile treatment.

Article Details

Keywords

Sperm DNA fragmentation, Intracytoplasmic sperm injection, sperm chromatin dispersion, blastocyst

References

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