Article Sidebar

PDF
Date Published: 07/08/2023
Abstract Views: 98
Views PDF: 92
DOI: https://doi.org/10.51298/vmj.v528i2.6097
Issue
Vol. 528 No. 2 (2023)
Section
Các bài báo
How to Cite
Bùi, N. L., Chu, Đình T., & Phùng , T. H. (2023). EVALUATING THE EFFECT OF DIFFERENT CONDITIONS ON MRNA EXPRESSION LEVELS OF MEST AND ADIPOGENESIS-RELATED GENES VIA MICROARRAY DATA. Vietnam Medical Journal, 528(2). https://doi.org/10.51298/vmj.v528i2.6097

EVALUATING THE EFFECT OF DIFFERENT CONDITIONS ON MRNA EXPRESSION LEVELS OF MEST AND ADIPOGENESIS-RELATED GENES VIA MICROARRAY DATA

Nhật Lệ Bùi1,2, Đình Tới Chu2, Thanh Hương Phùng 1,
1 Hanoi University of Pharmacy
2 International School, Vietnam National University, Hanoi

Main Article Content

Abstract

Mesoderm-specific transcript (Mest), which is assumed to be associated with the expansion of white adipose tissue, is predicted to be a biomarker and therapeutic target for obesity and other metabolic diseases. Thus, the genetic expression of Mest should be well-investigated for further application. In this study, we analyzed microarray data to evaluate the change in the mRNA expression level of the Mest gene and some genes related to adipogenesis in adipose tissue of 10-  and 21- day old mice raised at  17℃ and 29℃ ambient temperature. There were 839 genes, including Mest, having changes in their mRNA expression level with different ages, while this figure for different ambient temperatures was 11. From that, we found 41 differentially expressed genes (DEG) related to adipogenesis. The expression level of Mest dramatically declined about 40 to 60 times in 21-day-old mice compared with 10-day-old, while we found 36/41 adipogenesis-related DEG up-regulated. The Mest’s expression features in adipose tissue were similar to a cluster of 13 DEG, most of which were involved in the metabolic pathway.

Article Details

Keywords

Mest, adipogenesis, mRNA expression level

References

1. Jura, M., et al., Mest and Sfrp5 are biomarkers for healthy adipose tissue. Biochimie, 2016. 124: p. 124-133.
2. Kadota, Y., et al., Mouse mesoderm-specific transcript inhibits adipogenic differentiation and induces trans-differentiation into hepatocyte-like cells in 3T3-L1 preadiocytes. BMC Research Notes, 2022. 15(1): p. 164.
3. Yang, Y., S.J. Adelstein, and A.I. Kassis, Target discovery from data mining approaches. Drug Discovery Today, 2012. 17: p. S16-S23.
4. Rosell, M., et al., Brown and white adipose tissues: intrinsic differences in gene expression and response to cold exposure in mice. American Journal of Physiology-Endocrinology and Metabolism, 2014. 306(8): p. E945-E964.
5. Chu, D.-T., et al., C57BL/6J mice as a polygenic developmental model of diet-induced obesity. Physiological Reports, 2017. 5(7): p. e13093.
6. Chabowska-Kita, A., et al., Low ambient temperature during early postnatal development fails to cause a permanent induction of brown adipocytes. The FASEB Journal, 2015. 29(8): p. 3238-3252.
7. Chen, J.J., et al., Selection of differentially expressed genes in microarray data analysis. The Pharmacogenomics Journal, 2007. 7(3): p. 212-220.
8. Mayer, W., et al., Expression of the imprinted genes MEST/Mest in human and murine placenta suggests a role in angiogenesis. Developmental Dynamics, 2000. 217(1): p. 1-10.
9. Moreno-Mendez, E., et al., Early-life programming of adipose tissue. Nutrition Research Reviews, 2020. 33(2): p. 244-259.
10. Choi, M.-S., et al., High-fat diet decreases energy expenditure and expression of genes controlling lipid metabolism, mitochondrial function and skeletal system development in the adipose tissue, along with increased expression of extracellular matrix remodelling- and inflammation-related genes. British Journal of Nutrition, 2015. 113(6): p. 867-877.