Article Sidebar

PDF
Date Published: 07/08/2023
Abstract Views: 434
Views PDF: 234
DOI: https://doi.org/10.51298/vmj.v528i2.6108
Issue
Vol. 528 No. 2 (2023)
Section
Các bài báo
How to Cite
Hồng, T. K. N., & Phạm, T. B. P. (2023). SURVEY OF THE FREQUENCY OF BROAD-SPECTRUM Β-LACTAMASE-PRODUCING BACTERIA AND THEIR ANTIBIOTIC RESISTANCE AT BINH DAN HOSPITAL. Vietnam Medical Journal, 528(2). https://doi.org/10.51298/vmj.v528i2.6108

SURVEY OF THE FREQUENCY OF BROAD-SPECTRUM Β-LACTAMASE-PRODUCING BACTERIA AND THEIR ANTIBIOTIC RESISTANCE AT BINH DAN HOSPITAL

Thị Khánh Ngân Hồng1,, Thị Bích Phượng Phạm2
1 Binh Dan hospital
2 Ho Chi Minh City Institute of Public Health

Main Article Content

Abstract

Background: Previously, infections caused by E. coli, K. pneumoniae and Enterobacter could be easily treated with 3rd and 4th generation cephalosporins, now, these bacteria are resistant to nearly 70% of antibiotics, caused by bacteria capable of secreting broad-spectrum β-lactams (ESBL). Therefore, we surveyed the frequency of broad-spectrum β-lactamase-producing bacteria to contribute to the diagnosis and treatment of infectious diseases. Objective: Survey of the frequency of broad-spectrum β-lactamase-producing bacteria and their antibiotic resistance at Binh Dan hospital. Methods: Descriptive cross-sectional study, all specimens which were isolated including blood, pus, urine, and fluids then chosen in this study in 2018 to January 2020. Method for detecting antibiotic-resistant bacteria at the Microbiology Department of Binh Dan Hospital and ESBL-producing bacteria by Kirby Bauer tests and Modified Double Disc Synergy Test and Combined Disk Test. Result: The ESBL-producing gram-negative bacilli was 32.03%. Among 13,403 gram-negative bacilli isolated from 17,226 positive samples, there were 4,293 cultures with ESBL-producing gram-negative bacilli, mainly urine and pus. When surveying ESBL-producing samples, we found that the top 3 bacteria causing nosocomial infection were E. coli (39.49%); K.pneumonia (31.64%); Klebsiella spp. (22.63%). In addition, we also recorded 3 cases of P. mirabilis; 1 case of Serratia spp (pus). 1 case of Enterobacter cloacae and 1 case of A. baumannii (sputum). The rate of resistance of ESBL-producing bacteria to Quinolone, Cephalosporin, Aminoglycoside antibiotics are >50%. Klebsiella spp. more resistant to most antibiotics in hospital use than E.coli, such as antibiotics in combination with beta lactamase inhibitors, for Klebsiella spp. >70%, E. coli <20%; carbapenem group of E. coli <37%, while Klebsiella spp. <57%. Conclusion: This results of the study form the basis for further surveillance studies and strengthen the management for the reasonable use of antibiotics

Article Details

Keywords

ESBL, β-lactamase-producing bacteria, antibiotic resistance.

References

Chu Thị Hải Yến và cộng sự (2014). Khảo sát tỉ lệ đề kháng kháng sinh của vi khuẩn phân lập tại bệnh viện cấp cứu Trưng Vương. Y học Tp. Hồ Chí Minh, tập 18, số 5.
2. Lê Thị Thanh Thảo và cộng sự (2021). Khả năng tiết β-lactamase phổ rộng, carbapenamase của Escherichia coli và Klebsiella pneumoniae phân lập từ bệnh phẩm nước tiểu tại bệnh viện quận 2. Y học Tp. Hồ Chí Minh, tập 25, số 6, 62-68.
3. Romeo S. Gundran, et.al. (2019). Prevalence and distribution of blaCTX-M, blaSHV, blaTEM gens in extended- spectrum β- lactamase- producing E. coli isolates from broiler farms in the Philippines. BMC Veterinary Research volume 15, Article number: 227
4. Trà Anh Duy, Nguyễn Phúc Cẩm Hoàng (2014). Đánh giá đặc điểm dịch tễ học và kháng sinh đồ của vi khuẩn trong bệnh lý hẹp niệu. Y học Tp. Hồ Chí Minh, tập 18, số 1, 359-365.
5. Trần Lê Duy Anh và cộng sự (2016). Nhiễm khuẩn đường tiết niệu do vi khuẩn tiết ESBL tại bệnh viện Nhân Dân Gia Định: kết quả chẩn đoán và điều trị. Y học Tp. Hồ Chí Minh, tập 20, số 1, 85-91.
6. Yannick Caron, et.al. (2018). Beta-lactamase resistance among Enterobacteriacae in Cambodia: The four year itch. International Journal of Infectious Diseases, 66 (2018), 74-79.