NGHIÊN CỨU HOẠT TÍNH CHỐNG OXY HÓA, BẢO VỆ GAN CỦA CAO CHIẾT VÀ HELICTERES HIRSUTA LOUR

Abstract

Objective: Evaluation of the hepatoprotective antioxidant activity of Helicteres hirsuta L. extracts and screening of the inhibitory effects on cancer cells of compounds from H. hirsuta. Subjects, research methods: Extracts of HHM, HHW and HHE-1/1 of H. hirsuta L. collected in Thua Thien Hue. Evaluation of antioxidant activity on DPPH, MDA models and hepatoprotective activity. Evaluation of cancer cytotoxic and apoptosis-inducing activity of compounds on HepG2 cell line. Result: HHM, HHW and HHE-1/1  exhibit  activity of free radical scavenging DPPH with IC₅₀: 21.19-47.67 µg/mL, inhibition of membrane lipid peroxidation with IC₅₀: 20.20 - 26.58 µg/ml. Three extracts showed hepatoprotective capacity at concentrations of 100 and 20 µg/mL in vitro, in the model of liver injury by PAR, the AST and ALT activities of the administration extract groups were lower than the PAR group, the microscopic was hepatocellular degeneration compared with the of liver necrosis areas in the PAR group. Conclusion: HHM, HHW and HHE-1/1 had strong antioxidant activity on both DPPH and MDA models. Hepatoprotective activity was well demonstrated at concentrations of 100 and 20 µg/mL. The extracts were hepatoprotective, reducing elevated levels of AST, ALT as well as reducing microscopic damage in the liver..