ĐÁNH GIÁ TỔN THƯƠNG SIÊU VI THỂ NHU MÔ GAN Ở NGƯỜI VIỆT BỊ PHƠI NHIỄM CHẤT ĐỘC DIOXIN MẠN TÍNH TRÊN KÍNH HIỂN VI ĐIỆN TỬ QUÉT

Abstract

Objective: To evaluate the ultrastructural liver tissue damage in Vietnamese with chronic dioxin toxicity. Subjects and methods: This study used liver biopsy samples from 33 patients exposed to dioxin. The research method was cross-sectional and analyzed using scanning electron microscopy (SEM). The sample preparation process included washing and fixation with glutaraldehyde and osmic acid solution before gold plating and observation under SEM. Results: SEM observation showed images of liver tissue damage in all 33 cases. The damage was mainly due to the infiltration of inflammatory cells at different levels. The images showed the infiltration of fiber bands into the Disse space, the space between liver cells. At the same time, changes were observed in the ultrastructural morphology of mitochondria, and endoplasmic reticulum. These are typical liver tissue damage due to dioxin poisoning. Conclusion: Through SEM observation, chronic exposure to dioxin has caused liver tissue damage, especially in the Disse space and liver cells. This damage is mainly due to the infiltration of inflammatory cells and fiber bands at different levels, and there are changes in the ultrastructural morphology of liver cells. These are typical liver tissue damage due to dioxin poisoning.