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A VERIFICATION METHOD IN NEWBORNS SCREENING OF METABOLIC DISORDERS BY THE NEOBASE 2 MSMS KIT USING XEVO TQD
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Abstract
VerifiCation is required before implemeting a new test method in use. Linearity, preCision, aCCuraCy
eValutions were Carried out to Verify the performanCe of the Waters XEVO TQD MSMS system with the Neobase non-deriVatized MSMS Perkin Elmer kit for deteCting amino aCids and aCylCarnitine in newborns with metaboliC disorders. ACCeptanCe Criteria applied aCCording to CLSI - NBS04 2017 guidelines giVes the following results: Correlation CoeffiCient (r) ≥ 0,99 indiCates good linearity, within-run precisiOn CV ≤ 25%, between-run precisiOn CV ≤ 35%, recOvery is 40-140%. The results show that the linear range has the Correlation CoeffiCient from 0,9947 to 0,9998, the within-run preCision ranges from 2,83 – 10,12%, the between-run preCision ranges from 5,56 – 17,02%, reCoVery of 70,77 – 101,48% of metabolites Confirmed to be in aCCordanCe with the manufaCturer's Claim. Testing for Congenital metaboliC disorders by MSMS method is aCCurate and reliable in the aCtual Conditions of the testing Center - Gentis GenetiC SerViCes Joint StoCk Company, used in CliniCal praCtiCe to sCreen some Congenital metaboliC disorders
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Keywords
NBS04, XeVo TQD, Neobase2
References
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