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Date Published: 31/07/2021
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DOI: https://doi.org/10.51298/vmj.v502i2.657
Issue
Vol. 502 No. 2 (2021)
Section
Các bài báo
How to Cite
Ngọc Hoài Bảo, N. ., & Xuân Vĩnh, T. . (2021). EVALUATE THE EFFECTS OF 0,5% CHX GEL IN SITU ON THE VIABILITY AND MIGRATION OF HGFS. Vietnam Medical Journal, 502(2). https://doi.org/10.51298/vmj.v502i2.657

EVALUATE THE EFFECTS OF 0,5% CHX GEL IN SITU ON THE VIABILITY AND MIGRATION OF HGFS

Nguyễn Ngọc Hoài Bảo, Trần Xuân Vĩnh

Main Article Content

Abstract

Background: The gel in situ containing 0,5% chlorhexidine (CHX) is made by the Faculty of Pharmacy, University of Medicine and Pharmacy at Ho Chi Minh City. Human gingival fibroblast cells (hGFs) are one of major components of the gingival, playing an important role in wound healing. Objective: Evaluate the effects of 0,5% CHX gel in situ on the viability and migration of hGFs. Materials and Methods: The gel extract was diluted into 4 concentrations of 1/10, 1/102, 1/103 và 1/104. Cytotoxicity was assessed by MTT assay after incubation of hGFs for each concentration for 24 hours. Effect of safe concentrations on migration of hGFs was evaluated by scratch test. Results: Cells survival rate when exposed to 1/103 và 1/104 diluted CHX gel was over 70%. At safe concentrations, CHX gel in situ reduces the migration of hGFs. Conclusion: The effect of CHX gel in situ on hGFs depends on its diluted concentration and wiis similar to control gel.

Article Details

Keywords

Chlorhexidine, in situ gel, cytotoxicity, migration

References

1. Ban Zuhair A. (2020), "The Efficacy of Chlorhexidine Gel as an Adjunctive Treatment for Patient with Chronic Periodontitis", Indian Journal of Forensic Medicine & Toxicology. 14(1), pp. 544-550.
2. Garala K., Joshi P., Shah M., Ramkishan A. and Patel J. (2013), "Formulation and evaluation of periodontal in situ gel", Int J Pharm Investig. 3(1), pp. 29-41.
3. Nie S., Hsiao W. L., Pan W. and Yang Z. (2011), "Thermoreversible Pluronic F127-based hydrogel containing liposomes for the controlled delivery of paclitaxel: in vitro drug release, cell cytotoxicity, and uptake studies", Int J Nanomedicine. 6, pp. 151-66.
4. Li R., Guo W., Yang B., Guo L., Sheng L., Chen G., Li Y., Zou Q., Xie D., An X., Chen Y. and Tian W. (2011), "Human treated dentin matrix as a natural scaffold for complete human dentin tissue regeneration", Biomaterials. 32(20), pp. 4525-38.
5. Liang C. C., Park A. Y. and Guan J. L. (2007), "In vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro", Nat Protoc. 2(2), pp. 329-33.
6. Colombo M., Ceci M., Felisa E., Poggio C. and Pietrocola G. (2018), "Cytotoxicity evaluation of a new ozonized olive oil", European journal of dentistry. 12(4), pp. 585-589.
7. Sukumaran S. K., Vadakkekuttical R. J. and Kanakath H. (2020), "Comparative evaluation of the effect of curcumin and chlorhexidine on human fibroblast viability and migration: An in vitro study", J Indian Soc Periodontol. 24(2), pp. 109-116.
8. Mercan U., Gonen Z. B., Salkin H., Yalcin Ulker G. M. and Meral D. G. (2019), "Comparison of the effect of postoperative care agents on human gingival fibroblasts: a preliminary study", Eur Oral Res. 53(2), pp. 67-73.
9. Cabral C. T. and Fernandes M. H. (2007), "In vitro comparison of chlorhexidine and povidone-iodine on the long-term proliferation and functional activity of human alveolar bone cells", Clin Oral Investig. 11(2), pp. 64-155.