MỐI LIÊN QUAN GIỮA ĐỊNH DANH VI SINH VẬT GÂY BỆNH VIÊM NỘI TÂM MẠC NHIỄM TRÙNG BẰNG PHƯƠNG PHÁP REAL-TIME PCR VỚI MỘT SỐ ĐẶC ĐIỂM LÂM SÀNG, CẬN LÂM SÀNG

Abstract

Objectives: To evaluate the relationships between the results of identification of microorganisms causing infectious endocarditis (IE) by real-time PCR method with some clinical and subclinical features. Subjects and methods: A total of 108 patients were diagnosed as IE at the Vietnam Heart Institute - Bach Mai Hospital according to the ESC 2015 and tested by real-time PCR at 108 Clinical Medicine Research Institute. Clinical and laboratory characteristics including clinical symptoms, laboratory features including some parameters of echocardiography, hematological and blood biochemistry tests were collected. Real-tine PCR and blood culture procedures were performed to identify the pathogens. Results: The percentage of the patients presented symptoms of fever and chills in the group of the patients with positive PCR result test (76.7%) (PCR (+)) was significantly higher than in the group of the patients with negative PCR result test (PCR (-)) (57.1%) (OR: 2.471, p<0.05). The mean value for white blood cell number and blood level of highly sensitive C-reactive protein were  significantly higher in the group of the patients with PCR (+) than in the group of the patients with PCR (-) (p< 0.05). Real-time PCR results were  in agreement with the results of blood cultures test in determining Staphylococcus ssp. and Enterococus ssp. (p<0.05). Conclusion: The study illustrated the relationships between the results of identification of microorganisms causing infectious endocarditis (IE) by real-time PCR method and some clinical and subclinical symptoms. Using real-time PCR method showed high value and was in agreement with the results of blood culture test in identifying microorganisms causing IE.