RELATION BETWEEN IDENTIFICATION OF PATHOGENIC MICROORGANISMS CAUSING INFECTIVE ENDOCARDITIS USING REAL-TIME PCR METHOD AND SOME CLINICAL AND PARACLINICAL CHARACTERISTICS
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Abstract
Objectives: To evaluate the relationships between the results of identification of microorganisms causing infectious endocarditis (IE) by real-time PCR method with some clinical and subclinical features. Subjects and methods: A total of 108 patients were diagnosed as IE at the Vietnam Heart Institute - Bach Mai Hospital according to the ESC 2015 and tested by real-time PCR at 108 Clinical Medicine Research Institute. Clinical and laboratory characteristics including clinical symptoms, laboratory features including some parameters of echocardiography, hematological and blood biochemistry tests were collected. Real-tine PCR and blood culture procedures were performed to identify the pathogens. Results: The percentage of the patients presented symptoms of fever and chills in the group of the patients with positive PCR result test (76.7%) (PCR (+)) was significantly higher than in the group of the patients with negative PCR result test (PCR (-)) (57.1%) (OR: 2.471, p<0.05). The mean value for white blood cell number and blood level of highly sensitive C-reactive protein were significantly higher in the group of the patients with PCR (+) than in the group of the patients with PCR (-) (p< 0.05). Real-time PCR results were in agreement with the results of blood cultures test in determining Staphylococcus ssp. and Enterococus ssp. (p<0.05). Conclusion: The study illustrated the relationships between the results of identification of microorganisms causing infectious endocarditis (IE) by real-time PCR method and some clinical and subclinical symptoms. Using real-time PCR method showed high value and was in agreement with the results of blood culture test in identifying microorganisms causing IE.
Keywords
Infective endocarditis, echocardiography, blood culture, Real-Time PCR
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References
2. Bui STT, Duong HD, Vu TT, et al. Multimodality imaging in the diagnosis of bioprosthetic aortic valve endocarditis: A case report. Ann Med Surg (Lond). 2022; 80:104238
3. Ciliberto GR, Moreo A, Lobiati E, et al. The limitations of echocardiography in the overall diagnosis of the morphological lesions associated with infective endocarditis: comparison of echocardiographic and surgical findings. G Ital Cardiol. 1999; 29(12):1431-7.
4. Habib G, Lancellotti P, Antunes MJ, et al. 2015 ESC Guidelines for the management of infective endocarditis: The Task Force for the Management of Infective Endocarditis of the European Society of Cardiology (ESC). Endorsed by: European Association for Cardio-Thoracic Surgery (EACTS), the European Association of Nuclear Medicine (EANM) Eur. Heart J. 2015;36:3075–3128.
5. Halavaara M, Martelius T, Järvinen A, et al. Impact of pre-operative antimicrobial treatment on microbiological findings from endocardial specimens in infective endocarditis. Eur J Clin Microbiol Infect Dis. 2019; 38(3):497-503.
6. Liesman RM, Pritt BS, Maleszewski JJ, et al. Laboratory Diagnosis of Infective Endocarditis. Journal of Clinical Microbiology. 2017; 55, 2599–2608.
7. Murdoch DR, Corey GR, Hoen B, et al. International Collaboration on Endocarditis-Prospective Cohort Study (ICE-PCS) Investigators. Clinical presentation, etiology, and outcome of infective endocarditis in the 21st century: the International Collaboration on Endocarditis-Prospective Cohort Study. Arch Intern Med. 2009;169(5):463-73.
8. Yallowitz AW, Decker LC. Infectious Endocarditis. 2023. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2023 Jan–. PMID: 32491573.