TÁC NHÂN VI SINH PHÁT HIỆN TRÊN MẪU ĐÀM BỆNH NHÂN VIÊM PHỔI MẮC PHẢI CỘNG ĐỒNG NHẬP VIỆN

Abstract

Background: Hospitalized community - acquired pneumonia (CAP) is a common disease that usually caused by bacteria and virus. However, it is hard to identify the pathogens because of patient’s sputum, which gets contamination at the high risk when passing through the oropharynx. While the traditional culture for detecting pathogens is still limited, the multiplex real-time PCR is used as an optimal technique to detect not only the bacteria but also the atypical bacteria, virus and fungi. Objects: To determine the rate of pathogenic microorganisms in sputum of hospiatalized community - acquired pneumonia patients by multiplex real-time PCR. Methods: 138 samples of the sputum from CAP patients hospitalized at Nguyen Tri Phuong hospital were assessed the reliability based on Barlett’s scales before using multiplex real-time PCR to detect pathogenic microoganisms. Results: Among 138 sputum samples, there were 137 cases (99.3%) detected with pathogenic microoganisms, in which 9 cases were detected in single infection (6.5%) and 117 cases in co-infection (85.4%). There were 88.4% of sputum samples infected with bacteria, 79% infected with virus and 62.3% infected with fungi. Conclusions: The rate of pathogenic microoganisms detected from sputum of hospitalized CAP patients is 99.3%, in which bacterial infection 88.4%, viral infection 79.0% and fungi infection 62.3%. The rate of co-infection is 85.4%.