SO SÁNH HIỆU QUẢ GIỮA ĐÔNG LẠNH CHẬM VÀ THỦY TINH HÓA MÔ BUỒNG TRỨNG NGƯỜI VIỆT NAM DỰA TRÊN PHƯƠNG PHÁP KHẢO SÁT MÔ HỌC

Abstract

Freezing human ovarian tissue is primarily used to safeguard and restore the reproductive capabilities of cancer patients who are at high risk of infertility after radio- or chemotherapies. And it has been the sole way to maintain reproductive abilities prior to puberty. Controlled rate freezing (slow freezing) and vitrification are two main protocols in storing fertility. The objectives of this study are to evaluate and to compare the efficacy of two freezing protocols in Vietnamese ovarian tissue based on histological analysis via numbers of counted follicles between tissue segments of same donor. In this cross- sectional study, 24 ovarian tissues were obtained from 8 donors (27 ±3.78 years) were divided into three groups: fresh control group, slow freezing group and vitrification group. The integral composition of ovarian tissue that consists of primordial, primary and secondary follicles, remained unaltered following the freezing and warming procedures. In contrast to the fresh control group, the total number of counted follicles after two freezing methods was decreased, particularly the number of primary follicles, average 5.11± 4.14 follicles in slow freezing, 3.30 ± 6.40 follicles in vitrification, compared to 18.64 ± 15.34 follicles in fresh group, the differences had statistical significance (p<0.05). Notably, the proportion of primary follicles after vitrification (2.84 ± 2.83 %) was lower than that of fresh ones (13.84 ± 11.03 %), the disproportion was statistically significant (p<0.05). Both techniques could be able to store human ovarian tissue according to histological analysis, nevertheless slow freezing may be superior to vitrification.