XÂY DỰNG QUY TRÌNH ĐỊNH LƯỢNG ĐỒNG THỜI NARINGIN, NATRI BENZOAT VÀ KALI SORBAT TRONG VIÊN NANG BƯỞI NON VÀ ĐÁNH GIÁ ĐỘ HÒA TAN CỦA CHẾ PHẨM

Abstract

Objectives: To develop an analytical methodology to simultaneously determine naringin, sodium benzoate, and potassium sorbate in immature pomelo capsules and apply the procedure in the assessment of the solubility of product. Subjects and Methods: Naringin, sodium benzoate and potassium sorbate in immature pomelo capsules were determined by HPLC method. Results: Chromatographic separation was made with C₁₈ column using acetonitrile – acetic acid 0.1 % (20 : 80) as the mobile phase at 1,0 mL/min, column temperature of 35 ^oC, and detected at 235 nm. The process was validated the system suitsability; selectivity; linearity range; precision of both three compounds with RSD <2.0%; and accuracy with recovery ratio in 98 – 102 (%). The method of evaluating the solubility of immature pomelo capsules was developed with 0.1 M HCl as dissolution solvent. The solubility evaluation procedure was validated system suitability, selectivity, linearity range, precision, and accuracy. Conclusion: The process could be applied to determine the content of naringin, sodium benzoate, and potassium sorbate in immature pomelo capsules and evaluate the solubility of product.