NGHIÊN CỨU SỰ MẤT ỔN ĐỊNH VI VỆ TINH MỨC ĐỘ CAO VÀ MỐI LIÊN QUAN VỚI MỘT SỐ ĐẶC ĐIỂM MÔ BỆNH HỌC TRONG UNG THƯ BIỂU MÔ DẠ DÀY BẰNG PHƯƠNG PHÁP HÓA MÔ MIỄN DỊCH

Abstract

Objective: to investigate the prevalence of microsatellite instability (MSI) in patients with gastric carcinoma and its correlation with certain histopathological features. Subjects and methods: a retrospective and cross-sectional study was conducted on 87 gastric carcinoma patients at 103 Military Hospital from January 2020 to March 2023. Immunohistochemical staining with PMS2, MLH1, MSH2, and MSH6 immune markers was used to evaluate MSI. The obtained data were analyzed to assess the relationship between the different indicators. Results: 16.09% of the patients had MSI, with 13.79% of those exhibiting high-level MSI (MSI-H) and a loss of simultaneous expression of two immune markers (MLH1-PMS2) or all four immune markers. Additionally, 2.3% of the patients had low-level MSI (MSI-L) with a phenotype that only lost expression of the PMS2 marker. Statistical analysis demonstrated a significant association (p<0.05) between MSI-H and patients with Lauren intestinal type, moderate differentiation, invasion to the serosal fibrous connective tissue (pT3), high-grade Tumor-infiltrating lymphocytes, and tumor necrosis.