KHẢO SÁT GIÁ TRỊ CÁC DẤU ẤN HÓA SINH TRONG XÉT NGHIỆM SÀNG LỌC HỘI CHỨNG TRISOMY Ở THỜI GIAN VÀ NHIỆT ĐỘ BẢO QUẢN MẪU KHÁC NHAU

Abstract

Objective: To compare the median values of biochemical markers in prenatal screening for Trisomy syndrome between samples preserved under strict storage conditions and samples randomly transported from different sample-sending units.Subjects and methods: A longitudinal study was conducted with 50 test samples from pregnant women who underwent Double test screening at Ho Chi Minh City University of Medicine and Pharmacy Hospital 2, along with data from 515 Double test results of pregnant women performed at the STO Phuong Dong Hospital in Ho Chi Minh City, Vietnam (in 2022) stored in the Prisca software. The concentrations of PAPP-A and free β-hCG were analyzed, comparing the median values by gestational week of the first trimester with sample storage times of 24, 48, 72, and 96 hours. A comparison was also made with 50 samples collected and tested immediately within 4, 48, and 72 hours. PAPP-A and free β-hCG were quantified using the CLIA method on the Immulite 2000 system. Statistical analysis was performed using SPSS.Results: There was no significant difference in the median value of PAPP-A between the preserved and strictly temperature-monitored sample group compared to the data group. The concentration of free β-hCG was significantly higher (49%) in the data group compared to the control group. The concentration of free β-hCG also increased when the sample was stored at room temperature for 48 hours. Conclusion: The median values of PAPP-A and free β-hCG remained stable up to 72 hours in the group with time and temperature monitoring. The concentration of free β-hCG was higher in the randomly transported sample group compared to the control group. Storing the test samples at a temperature above 20 degrees Celsius increased the concentration of free β-hCG at a 48-hour storage time.