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EMBRYO MOSAIC: SOME FACTORS RELATED TO THE MOSAICISM IN BLASTOCYSTS
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Abstract
Objective: This study aimed to determine the mosaic rates according to classifications in blastocyst and to analyze some factors related to the mosaicism status in 655 blastocysts from 198 couples undergoing in vitro fertilization (IVF) preimplantation genetic testing using next-generation sequencing (NGS). Method: A retrospective cross-sectional, utilizing available data at the Tam Anh General Hospital's Reproductive Support Center in Hanoi, from March 2021 to February 2022. Results: The rate of mosaic embryos was 27.48%; mosaicism of 1 pair of chromosomes (Chr), 2 pairs of Chr, and from 3 pairs of Chr upwards were at the rates of 11.76%; 7.32%, and 8.40%, respectively. Chromosome aneuploidy mosaics accounted for 20.46% of cases, while structural mosaicism was 3.81%, and mixed mosaicism was 3.21%. The low-level mosaic rate was 11.30%, and the high-level mosaic rate was 16.18%. High-level mosaicism in trophectoderm (TE) grade B was 3.06 times significantly higher than that in TE grade A, with statistical significance at p<0.005. No association was found between clinical features including maternal age, paternal age, body mass index, number of days of ovarian stimulation, total gonadotropin dose, and other morphological features of the embryos with the mosaic rate, as well as the risk of the embryos' mosaicism levels. Conclusion: Trophectoderm morphology can be related to the level of mosaicism in the blastocyst embryos before implantation.
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Keywords
Blastocyst, mosaic embryo, incidence of mosaicism, PGT-A, NGS.
References
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