NGHIÊN CỨU ĐỊNH LƯỢNG SARS-COV-2 BẰNG PHƯƠNG PHÁP REALTIME-PCR SỬ DỤNG CHẤT NHUỘM SYBR GREEN

Abstract

The COVID-19 pandemic caused by the SARS-CoV-2 virus has spread across countries around the world with an increasing number of infections and deaths. Quantitative testing of SARS-CoV-2 has a particularly important strategic role, is widely used and is considered the "gold standard" in helping doctors diagnose and properly assess the disease condition. Especially for patients with many underlying diseases and weak immune health, the correct assessment of the stage and progression of the disease makes a great contribution to timely prognosis and treatment. However, current quantitative tests are expensive, posing a challenge for authorities to find new, cheaper solutions that still retain similar specificity and accuracy. equivalent, helping to reduce the economic burden on the community and society. Therefore, we conduct quantitative study of SARS-CoV-2 by Realtime-PCR method, specifically Realtime-PCR method using SYBR Green I dye to validate and confirm the new method has specificity High performance and accuracy at a lower cost. The study was carried out based on the C(+) control provided from the manufacturer and was verified on real samples according to laboratory procedures. The results will be collected and processed to evaluate the precision, accuracy and build the calibration curve. The results show that the Realtime-PCR method using SYBR Green I dye can quantify SARS-CoV-2 virus with high specificity and accuracy.