RESEARCH IDENTIFIED SOME GENE MUTATIONS THAT CAUSE HYPERTENSION BY SANGER GENE SEQUENCING METHOD
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Abstract
Hypertension (HTN) is one of the biggest issues for global public health today, not only for development countries but also for developing countries, especially HTN is getting younger and younger. With unpredictable complications, hypertension increases the mortality rate, increases the rate of disability and affects the patient's life. In which, up to 95% is due to idiopathic hypertension. This study aims to identify some single nucleotide polymorphisms (SNPs) that cause the risk of idiopathic hypertension. Objectives: Study of 4 SNPs with the highest frequency of variants on the CYP3A, NOS3 and GNB3 genes, including the CYP3A5 – 6096A-G (c.7081 A>G); NOS3 – E298D (c.8007 T>G); NOS3-786T-C (c.1975 C>T) and GNB3-825C-T (c.4787 C>T) by Sanger sequencing. Samples of 5 patients with hypertension of unknown cause are used in this study. Methods: A cross-sectional descriptive study. Results: The CYP3A5 – 6096A-G with homozygous genotype was detected in all 5 patients. The NOS3 – E298D (c.8007 T>G) SNP was identified in 3 patients with homozygous genotype and 1 patient with homozygous genotype. The NOS3-786T-C was found in all 5 patients with rate of 4:1 (homozygous:heterozygous). The result also indicated 3 patients with heterozygous genotype and 1 patient with homozygous genotype of GNB3-825C-T SNP.
Article Details
Keywords
Hypertension, CYP3A5, NOS3, GNB3
References
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