OPTIMIZATION OF ANTIBODIES ONTO THE AU NANOPARTICLES TO ENHANCE THE SENSITIVITY OF LATERAL FLOW IMMUNOASSAY FOR DETECTION OF AFLATOXIN B1
Main Article Content
Abstract
Objectives: To enhance the sensitivity of Lateral flow immunoassay for the detection of mycotoxins Aflatoxin B1 in food through optimization of key parameters. Methods: Optimal parameters include: concentration of antibody binding to gold nanoparticles, design of width of Lateral Flow Immunoassay, selection of nitrocellulose membranes, variation of flow rate. Results: Optimal antibody concentration is 45 µg/ml, Borat buffer pH 9, volume of buffer solution is 0,7 ml in 120 µl OD 30 gold nanoparticles. Test strip width is 0,3 cm and nitrocellullose membrane type FF120HP. Conclusions: By optimizing the important parameters affecting the color signal intensity on the test line, we have improved the sensitivity of the Lateral Flow Immunoassay.
Keywords
Lateral Flow Immunoassay, LFIA, rapid detection, Aflatoxin B1, sensitivity
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References
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