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Date Published: 24/11/2023
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DOI: https://doi.org/10.51298/vmj.v532i1.7293
Issue
Vol. 532 No. 1 (2023)
Section
Các bài báo
How to Cite
Nguyễn, V. C., Nguyễn, T. T. T., Hoàng, T. T., Chu, Đức T. ., Nguyễn, V. B., Lê, T. A., & Nguyễn, T. Đạt. (2023). OPTIMIZATION OF ANTIBODIES ONTO THE AU NANOPARTICLES TO ENHANCE THE SENSITIVITY OF LATERAL FLOW IMMUNOASSAY FOR DETECTION OF AFLATOXIN B1. Vietnam Medical Journal, 532(1). https://doi.org/10.51298/vmj.v532i1.7293

OPTIMIZATION OF ANTIBODIES ONTO THE AU NANOPARTICLES TO ENHANCE THE SENSITIVITY OF LATERAL FLOW IMMUNOASSAY FOR DETECTION OF AFLATOXIN B1

Văn Chuyên Nguyễn1,, Thị Thu Trang Nguyễn1, Thị Trường Hoàng1, Đức Tiến Chu1, Văn Ba Nguyễn1, Tuấn Anh Lê1, Trọng Đạt Nguyễn2
1 Military Medical University
2 Military Institute of Preventive Medicine

Main Article Content

Abstract

Objectives: To enhance the sensitivity of Lateral flow immunoassay for the detection of mycotoxins Aflatoxin B1 in food through optimization of key parameters. Methods: Optimal parameters include: concentration of antibody binding to gold nanoparticles, design of width of Lateral Flow Immunoassay, selection of nitrocellulose membranes, variation of flow rate. Results: Optimal antibody concentration is 45 µg/ml, Borat buffer pH 9, volume of buffer solution is 0,7 ml in 120 µl OD 30 gold nanoparticles. Test strip width is 0,3 cm and nitrocellullose membrane type FF120HP. Conclusions: By optimizing the important parameters affecting the color signal intensity on the test line, we have improved the sensitivity of the Lateral Flow Immunoassay.

Article Details

Keywords

Lateral Flow Immunoassay, LFIA, rapid detection, Aflatoxin B1, sensitivity

References

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